Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone

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dc.contributor.authorPeltomaa, Riikka Johanna
dc.contributor.authorFarka, Zdeněk
dc.contributor.authorMickert, Matthias
dc.contributor.authorBrandmeier, Julian
dc.contributor.authorPastucha, Matěj
dc.contributor.authorHlaváček, Antonín
dc.contributor.authorMartínez Orts, Mónica
dc.contributor.authorCanales Mayordomo, María Ángeles
dc.contributor.authorSkládal, Petr
dc.contributor.authorBenito Peña, María Elena
dc.contributor.authorMoreno Bondi, María Cruz
dc.contributor.authorGorris, Hans
dc.date.accessioned2024-02-12T08:55:27Z
dc.date.available2024-02-12T08:55:27Z
dc.date.issued2020
dc.description.abstractDue to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL−1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (α- and β-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.identifier.citationPeltomaa, Riikka, et al. «Competitive Upconversion-Linked Immunoassay Using Peptide Mimetics for the Detection of the Mycotoxin Zearalenone». Biosensors and Bioelectronics, vol. 170, diciembre de 2020, p. 112683. https://doi.org/10.1016/j.bios.2020.112683.
dc.identifier.doi10.1016/j.bios.2020.112683
dc.identifier.officialurlhttps://www.doi.org/10.1016/j.bios.2020.112683
dc.identifier.urihttps://hdl.handle.net/20.500.14352/101131
dc.journal.titleBiosensors and Bioelectronics
dc.language.isoeng
dc.page.initial112683
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu546
dc.subject.keywordZearalenone
dc.subject.keywordUpconversion nanoparticle
dc.subject.keywordPeptide mimetic
dc.subject.keywordImmunosensing
dc.subject.keywordSurface plasmon resonance
dc.subject.keywordFood safety
dc.subject.ucmCiencias
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco23 Química
dc.subject.unesco24 Ciencias de la Vida
dc.titleCompetitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone
dc.typejournal article
dc.type.hasVersionP
dc.volume.number170
dspace.entity.typePublication
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