Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone
dc.contributor.author | Peltomaa, Riikka Johanna | |
dc.contributor.author | Farka, Zdeněk | |
dc.contributor.author | Mickert, Matthias | |
dc.contributor.author | Brandmeier, Julian | |
dc.contributor.author | Pastucha, Matěj | |
dc.contributor.author | Hlaváček, Antonín | |
dc.contributor.author | Martínez Orts, Mónica | |
dc.contributor.author | Canales Mayordomo, María Ángeles | |
dc.contributor.author | Skládal, Petr | |
dc.contributor.author | Benito Peña, María Elena | |
dc.contributor.author | Moreno Bondi, María Cruz | |
dc.contributor.author | Gorris, Hans | |
dc.date.accessioned | 2024-02-12T08:55:27Z | |
dc.date.available | 2024-02-12T08:55:27Z | |
dc.date.issued | 2020 | |
dc.description.abstract | Due to increasing food safety standards, the analysis of mycotoxins has become essential in the food industry. In this work, we have developed a competitive upconversion-linked immunosorbent assay (ULISA) for the analysis of zearalenone (ZEA), one of the most frequently encountered mycotoxins in food worldwide. Instead of a toxin-conjugate conventionally used in competitive immunoassays, we designed a ZEA mimicking peptide extended by a biotin-linker and confirmed its excellent suitability to mimic ZEA by nuclear magnetic resonance (NMR) and surface plasmon resonance (SPR) analysis. Upconversion nanoparticles (UCNP, type NaYF4:Yb,Tm) served as background-free optical label for the detection of the peptide mimetic in the competitive ULISA. Streptavidin-conjugated UCNPs were prepared by click reaction using an alkyne-PEG-neridronate linker. The UCNP conjugate clearly outperformed conventional labels such as enzymes or fluorescent dyes. With a limit of detection of 20 pg mL−1 (63 pM), the competitive ULISA is well applicable to the detection of ZEA at the levels set by the European legislation. Moreover, the ULISA is specific for ZEA and its metabolites (α- and β-zearalenol) without significant cross-reactivity with other related mycotoxins. We detected ZEA in spiked and naturally contaminated maize samples using liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) as a reference method to demonstrate food analysis in real samples. | |
dc.description.department | Depto. de Química Analítica | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Universidad Complutense de Madrid | |
dc.description.sponsorship | Ministerio de Economía y Competitividad (España) | |
dc.description.status | pub | |
dc.identifier.citation | Peltomaa, Riikka, et al. «Competitive Upconversion-Linked Immunoassay Using Peptide Mimetics for the Detection of the Mycotoxin Zearalenone». Biosensors and Bioelectronics, vol. 170, diciembre de 2020, p. 112683. https://doi.org/10.1016/j.bios.2020.112683. | |
dc.identifier.doi | 10.1016/j.bios.2020.112683 | |
dc.identifier.officialurl | https://www.doi.org/10.1016/j.bios.2020.112683 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/101131 | |
dc.journal.title | Biosensors and Bioelectronics | |
dc.language.iso | eng | |
dc.page.initial | 112683 | |
dc.publisher | Elsevier | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.cdu | 546 | |
dc.subject.keyword | Zearalenone | |
dc.subject.keyword | Upconversion nanoparticle | |
dc.subject.keyword | Peptide mimetic | |
dc.subject.keyword | Immunosensing | |
dc.subject.keyword | Surface plasmon resonance | |
dc.subject.keyword | Food safety | |
dc.subject.ucm | Ciencias | |
dc.subject.ucm | Ciencias Biomédicas | |
dc.subject.unesco | 23 Química | |
dc.subject.unesco | 24 Ciencias de la Vida | |
dc.title | Competitive upconversion-linked immunoassay using peptide mimetics for the detection of the mycotoxin zearalenone | |
dc.type | journal article | |
dc.type.hasVersion | P | |
dc.volume.number | 170 | |
dspace.entity.type | Publication | |
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relation.isAuthorOfPublication | 8766057b-6628-4a02-a6db-20bddfaf3054 | |
relation.isAuthorOfPublication.latestForDiscovery | ebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85 |
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