A next-generation sequencing approach for the detection of mixed species in canned tuna

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dc.contributor.authorKlapper, Regina
dc.contributor.authorVelasco, Amaya
dc.contributor.authorDöring, Maik
dc.contributor.authorSchröder, Ute
dc.contributor.authorSotelo, Carmen G.
dc.contributor.authorBrinks, Erik
dc.contributor.authorMuñoz Colmenero, Ana Marta
dc.date.accessioned2024-11-05T19:21:15Z
dc.date.available2024-11-05T19:21:15Z
dc.date.issued2023
dc.descriptionThis work was carried out with funding from the EU Interreg Atlantic Area programme, project “SEATRACES”, number EAPA_87/2016.
dc.description.abstractTuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region markers has been tested. Analyses of defined mixtures of DNA, fresh tissue and canned tissue revealed a qualitative and, to some extent, semiquantitative identification of tuna species. While the choice of the bioinformatic pipeline had no influence in the results (p = 0.71), quantitative differences occurred depending on the treatment of the sample, marker, species, and mixture (p < 0.01). The results revealed that matrix-specific calibrators or normalization models should also be used in NGS. The method represents an important step towards a semiquantitative method for routine control of this analytically challenging food matrix. Tests of commercial samples uncovered mixed species in some cans, being not in compliance with EU regulations.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Commission
dc.description.statuspub
dc.identifier.citationKlapper, R., Velasco, A., Döring, M., Schröder, U., Sotelo, C. G., Brinks, E., & Muñoz-Colmenero, M. (2023). A next-generation sequencing approach for the detection of mixed species in canned tuna. Food Chemistry: X, 17. https://doi.org/10.1016/J.FOCHX.2023.100560
dc.identifier.doi10.1016/j.fochx.2023.100560
dc.identifier.issn2590-1575
dc.identifier.officialurlhttps://doi.org/10.1016/j.fochx.2023.100560
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S2590157523000020?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/110043
dc.journal.titleFood Chemistry X
dc.language.isoeng
dc.page.final12
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/Interreg Atlantic Area programme/EAPA_87%2F2016/EU/[SEATRACES]
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu579.6
dc.subject.cdu641.1.012.7
dc.subject.cdu664.95
dc.subject.cdu575.113
dc.subject.keywordAmplicon sequencing
dc.subject.keywordThunnus species identification
dc.subject.keywordSeafood
dc.subject.keywordFood fraud
dc.subject.keywordNGS
dc.subject.keywordTuna cans
dc.subject.ucmTecnología de los alimentos
dc.subject.ucmBiotecnología
dc.subject.ucmGenética
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.titleA next-generation sequencing approach for the detection of mixed species in canned tuna
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number17
dspace.entity.typePublication
relation.isAuthorOfPublicatione88ee515-1da2-4021-9c3b-b6f1ff423a51
relation.isAuthorOfPublication.latestForDiscoverye88ee515-1da2-4021-9c3b-b6f1ff423a51

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