Cu-doped hollow bioactive glass nanoparticles for bone infec-tion treatment

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dc.contributor.authorJiménez Holguín, Javier
dc.contributor.authorSánchez Salcedo, Sandra
dc.contributor.authorCicuéndez Maroto, Mónica
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.contributor.authorSalinas Sánchez, Antonio J.
dc.date.accessioned2023-06-22T12:28:57Z
dc.date.available2023-06-22T12:28:57Z
dc.date.issued2022-04-12
dc.descriptionRESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí) RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez) ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)
dc.description.abstractIn search of new approaches to treat bone infection and prevent drug resistance development, a nanosystem based on hollow bioactive glass nanoparticles (HBGN) of composition 79.5SiO2–(18-x)CaO–2.5P2O5–xCuO (x = 0, 2.5 or 5 mol-% CuO) was developed. The objective of the study was to evaluate the capacity of the HBGN to be used as nanocarriers of the broad-spectrum anti-biotic danofloxacin and source of bactericidal Cu2+ ions. Core-shell nanoparticles with specific surface areas close to 800 m2/g and pore volumes around 1 cm3/g were obtained by using hexa-decyltrimethylammonium bromide (CTAB) and poly (styrene)-block-poly (acrylic acid) (PS-b-PAA) as structure-directing agents. Flow cytometry studies showed the cytocompatibility of the nanoparticles in MC3T3-E1 pre-osteoblastic cell cultures. Ion release studies confirmed the release of non-cytotoxic concentrations of Cu2+ ions within the therapeutic range. Moreover, it was shown that the inclusion of copper in the system resulted in a more gradual release of da-nofloxacin that was extended over one week. The bactericidal activity of the nanosystem was evaluated with E. coli and S. aureus strains. Nanoparticles with copper were not able to reduce bacterial viability by themselves and Cu-free HBGN failed to reduce bacterial growth, despite releasing higher antibiotic concentrations. However, HBGN enriched with copper and da-nofloxacin drastically reduced bacterial growth in sessile, planktonic and biofilm states, which was attributed to a synergistic effect between the action of Cu2+ ions and danofloxacin. There-fore, the nanosystem here investigated is a promising candidate as an alternative for the local treatment of bone infections.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. Horizonte 2020
dc.description.sponsorshipInstituto de Salud Carlos III (ISCIII)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/75488
dc.identifier.doi10.3390/pharmaceutics14040845
dc.identifier.issn1999-4923
dc.identifier.officialurlhttps://doi.org/10.3390/pharmaceutics14040845
dc.identifier.relatedurlhttps://www.ucm.es/valletregigroup
dc.identifier.urihttps://hdl.handle.net/20.500.14352/72637
dc.journal.titlePharmaceutics
dc.language.isoeng
dc.page.initial845
dc.publisherMDPI
dc.relation.projectIDVERDI (694160)
dc.relation.projectIDPI20/01384
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu615.546
dc.subject.cdu546
dc.subject.keywordHollow nanoparticles
dc.subject.keywordMesoporous glasses
dc.subject.keywordCopper
dc.subject.keywordAntibacterial infection pre-osteoblasts
dc.subject.ucmMateriales
dc.subject.ucmQuímica inorgánica (Farmacia)
dc.subject.unesco3312 Tecnología de Materiales
dc.titleCu-doped hollow bioactive glass nanoparticles for bone infec-tion treatment
dc.typejournal article
dc.volume.number14
dspace.entity.typePublication
relation.isAuthorOfPublication82cf0840-5331-47de-a3ab-797fab8b9132
relation.isAuthorOfPublication14ed7f4d-b114-4a3c-9d8c-83f05fbfc703
relation.isAuthorOfPublication94b23d40-3b2e-4dad-b72d-96c864251f14
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relation.isAuthorOfPublication.latestForDiscovery82cf0840-5331-47de-a3ab-797fab8b9132
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