Dextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality

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dc.contributor.authorGao, Shipeng
dc.contributor.authorTorrente Rodríguez, Rebeca M.
dc.contributor.authorPedrero Muñoz, María
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorRocha Martin, Javier
dc.contributor.authorGuisán, José M.
dc.date.accessioned2023-06-22T10:43:12Z
dc.date.available2023-06-22T10:43:12Z
dc.date.issued2022-05-21
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2022)
dc.description.abstractMagnetic nanoparticles (MNPs) can be used as antibody carriers in a wide range of immunosensing applications. The conjugation chemistry for preparing antibody-MNP bionanohybrids should assure the nanoparticle’s colloidal dispersity, directional conformation and high biofunctionality retention of attached antibodies. In this work, peroxidase (HRP) was selected as model target analyte, and stable antibody-MNP conjugates were prepared using polyaldehyde-dextrans as multivalent linkers, also to prevent nanoparticles agglomeration and steric shielding of non-specific proteins. Under the manipulation of the oxidation variables, MNP-conjugated antibody showed the highest Fab accessibility, of 1.32 μmol analyte per μmol antibody, corresponding to 139 μmol aldehyde per gram of nanocarrier (5 mM NaIO4, 4 h). Demonstrating anti-interference advantage up to 10% serum, colorimetric immunoassay gave a detection limit (LOD) of 300 ng mL− 1 , while electrochemical transduction led to a considerable (680 times) improvement, with a LOD of 0.44 ng mL− 1 . In addition, polyaldehydedextran showed priority over polycarboxylated-dextran as the multivalent antibody crosslinker for MNPs in terms of sensitivity and LOD value, while immunosensors constructed with carboxylated magnetic microbeads (HOOC-MBs) outperformed MNPs-based immunoplatforms. This work sheds light on the importance of surface chemistry (type and density of functional groups) and the dimension (nanosize vs micrometer) of magnetic carriers to conjugate antibodies with better directional orientation and improve the analytical performance of the resulting immunosensors.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Biológicas
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipChina Scholarship Council
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/72449
dc.identifier.doi10.1016/j.talanta.2022.123549
dc.identifier.issn0039-9140
dc.identifier.officialurlhttps://doi.org/10.1016/j.talanta.2022.123549
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S0039914022003459#ack0010
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71496
dc.journal.titleTalanta
dc.language.isoeng
dc.page.initial123549
dc.publisherElsevier
dc.relation.projectID(PID2019-103899RB-I00)
dc.relation.projectIDTRANSNANOAVANSENS-CM Program (S2018/NMT-4349); (IND2018/ BIO-9480)
dc.relation.projectID(201808440415)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu577.1
dc.subject.cdu539.2:620.1
dc.subject.keywordIron oxide nanoparticles
dc.subject.keywordDextran-coated nanoparticles
dc.subject.keywordAntibody orientation
dc.subject.keywordImmunosensors
dc.subject.ucmBioquímica (Química)
dc.subject.ucmQuímica analítica (Química)
dc.subject.ucmBiotecnología
dc.subject.unesco2301 Química Analítica
dc.subject.unesco3399 Otras Especialidades Tecnológicas
dc.titleDextran-coated nanoparticles as immunosensing platforms: Consideration of polyaldehyde density, nanoparticle size and functionality
dc.typejournal article
dc.volume.number247
dspace.entity.typePublication
relation.isAuthorOfPublication422f8844-54d1-4615-98e8-ef5a0c7e3519
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication6945fb1e-9c48-459d-b994-e64328c3e79b
relation.isAuthorOfPublication.latestForDiscovery422f8844-54d1-4615-98e8-ef5a0c7e3519
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