Intravenous Lidocaine Decreases Tumor Necrosis Factor Alpha Expression Both Locally and Systemically in Pigs Undergoing Lung Resection Surgery

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dc.contributor.authorGarutti Martínez, Ignacio
dc.contributor.authorRancán, Lisa
dc.contributor.authorSimón Adiego, Carlos María
dc.contributor.authorLópez Gil, María Teresa
dc.contributor.authorCusati, Gabriel
dc.contributor.authorSanchez-Pedrosa, Guillermo
dc.contributor.authorMoraga, Francisco
dc.contributor.authorOlmedilla, Luis
dc.contributor.authorVara Ameigeiras, Elena María
dc.date.accessioned2024-01-15T16:43:51Z
dc.date.available2024-01-15T16:43:51Z
dc.date.issued2014
dc.description.abstractBackground: Lung resection surgery is associated with an inflammatory reaction. The use of 1-lung ventilation (OLV) seems to increase the likelihood of this reaction. Different prophylactic and therapeutic measures have been investigated to prevent lung injury secondary to OLV. Lidocaine, a commonly used local anesthetic drug, has antiinflammatory activity. Our main goal in this study was to investigate the effect of IV lidocaine on tumor necrosis factor α (TNF-α) lung expression during lung resection surgery with OLV. Methods: Eighteen pigs underwent left caudal lobectomy. The animals were divided into 3 groups: control, lidocaine, and sham. All animals received general anesthesia. In addition, animals in the lidocaine group received a continuous IV infusion of lidocaine during surgery (1.5 mg/kg/h). Animals in the sham group only underwent thoracotomy. Samples of bronchoalveolar lavage (BAL) fluid and plasma were collected before initiation of OLV, at the end of OLV, at the end of surgery, and 24 hours after surgery. Lung biopsy specimens were collected from the left caudal lobe (baseline) before surgery and from the mediastinal lobe and the left cranial lobe 24 hours after surgery. Samples were flash-frozen and stored to measure levels of the following inflammatory markers: interleukin (IL) 1β, IL-2, IL-10, TNF-α, nuclear factor κB, monocyte chemoattractant protein-1, inducible nitric oxide synthase, and endothelial nitric oxide synthase. Markers of apoptosis (caspase 3, caspase 9, Bad, Bax, and Bcl-2) were also measured. In addition, levels of metalloproteinases and nitric oxide metabolites were determined in BAL fluid and in plasma samples. A nonparametric test was used to examine statistical significance. Results: OLV caused lung damage with increased TNF-α expression in BAL, plasma, and lung samples. Other inflammatory (IL-1β, nuclear factor κB, monocyte chemoattractant protein-1) and apoptosis (caspase 3, caspase 9, and BAX) markers were also increased. With the use of IV lidocaine there was a significant decrease in the levels of TNF-α in the same samples compared with the control group. Lidocaine administration also reduced the inflammatory and apoptotic changes observed in the control group. Hemodynamic values, blood gas values, and airway pressure were similar in all groups. Conclusions: Our results suggest that lidocaine can prevent OLV-induced lung injury through reduced expression of proinflammatory cytokines and lung apoptosis. Administration of lidocaine may help to prevent lung injury during lung surgery with OLV.en
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.sponsorshipFundación Mutua Madrileña
dc.description.sponsorshipFundación Rodríguez Pascual
dc.description.sponsorshipSociedad Madrileña de Neumología y Cirugía Torácica
dc.description.statuspub
dc.identifier.citationGarutti I, Rancan L, Simón C, Cusati G, Sanchez-Pedrosa G, Moraga F, Olmedilla L, Lopez-Gil MT, Vara E. Intravenous lidocaine decreases tumor necrosis factor alpha expression both locally and systemically in pigs undergoing lung resection surgery. Anesth Analg. 2014 Oct;119(4):815-828. doi: 10.1213/ANE.0000000000000360. PMID: 25036372.
dc.identifier.doi10.1213/ane.0000000000000360
dc.identifier.issn0003-2999
dc.identifier.officialurlhttps://doi.org/10.1213/ane.0000000000000360
dc.identifier.pmid25036372
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/25036372/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93185
dc.issue.number4
dc.journal.titleAnesthesia & Analgesia
dc.language.isoeng
dc.page.final828
dc.page.initial815
dc.publisherLippincott, Williams & Wilkins
dc.relation.projectIDPI10/00986
dc.relation.projectIDPI10/0900
dc.relation.projectIDAP101072012
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.jel617-089.5
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco2403 Bioquímica
dc.titleIntravenous Lidocaine Decreases Tumor Necrosis Factor Alpha Expression Both Locally and Systemically in Pigs Undergoing Lung Resection Surgeryen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number119
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