Serological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests

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dc.contributor.authorÁlvarez García, Gema
dc.contributor.authorGarcía Culebras, Alicia
dc.contributor.authorGutiérrez-Expósito, Daniel
dc.contributor.authorNavarro-Lozano, Vanesa
dc.contributor.authorPastor Fernández, Iván
dc.contributor.authorOrtega Mora, Luis Miguel
dc.date.accessioned2023-12-15T18:38:11Z
dc.date.available2023-12-15T18:38:11Z
dc.date.issued2013-11-15
dc.description.abstractBovine neosporosis control programs are currently based on herd management and serodiagnosis because effective treatments and vaccines are unavailable. Although a wide variety of serological tools have been developed, enzyme-linked immunosorbent assays (ELISAs) are the most commonly commercialized tests. Partial comparative studies have been performed in the past, and the panel of available ELISAs has notably changed in the last few years. Therefore, diagnostic laboratories are requesting updated information about the performance of these tests. Accordingly, the aim of this study was to compare all of the commercially available ELISAs (n = 10) by evaluating their performance and to re-standardize them based on TG-ROC analyses when necessary. For this purpose, a well-characterized serum panel from experimentally and naturally infected bovines and non-infected bovines (n = 458) was used. Two different definitions of gold standard were considered: (i) the result of the majority of tests and (ii) pre-test information based on epidemiological, clinical and serological data. Most of the tests displayed high sensitivity (Se) and specificity (Sp) values when both gold standard criteria were considered. Furthermore, all the tests showed near perfect agreement, with the exception of the pair-wise comparisons that included the VMRD and SVANOVIR. The best-adjusted ELISAs were the HIPRA-CIVTEST, IDVET, BIOVET and IDEXX Rum (Se and Sp > 95%). After the TG-ROC analyses, higher Se and Sp values were obtained for the BIO-X, LSI Bov, LSI Rum and IDEXX Bov, though the increases were more significant for the SVANOVIR and VMRD. The Kappa values also increased with the new adjusted cut-offs. This is the first study that offers updated performance evaluations of commercially available ELISAs. Such analyses are essential for diagnostic laboratories and are valuable to the companies that develop and distribute these tests.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.doi10.1016/j.vetpar.2013.07.033
dc.identifier.essn1873-2550
dc.identifier.issn0304-4017
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S030440171300438X?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91381
dc.issue.number1-2
dc.journal.titleVeterinary Parasitology
dc.language.isoeng
dc.page.final95
dc.page.initial85
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordBovine neosporosis
dc.subject.keywordCommercial ELISAs
dc.subject.keywordComparative study
dc.subject.keywordDiagnostic performance
dc.subject.keywordAgreement
dc.subject.keywordTG-ROC
dc.subject.ucmParasitología (Veterinaria)
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleSerological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number198
dspace.entity.typePublication
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