The cytotoxin α‐sarcin behaves as a cyclizing ribonuclease

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dc.contributor.authorLacadena García-Gallo, Francisco Javier
dc.contributor.authorMartínez Del Pozo, Álvaro
dc.contributor.authorValle Lacadena
dc.contributor.authorMartínez Ruiz, Antonio
dc.contributor.authorMancheño Gómez, José Miguel
dc.contributor.authorOñaderra Sánchez, Mercedes
dc.contributor.authorGavilanes Franco, José Gregorio
dc.date.accessioned2024-04-23T11:00:03Z
dc.date.available2024-04-23T11:00:03Z
dc.date.issued1998-03-06
dc.description.abstractThe hydrolysis of adenylyl(3PC5P)adenosine (ApA) and guanylyl(3PC5P)adenosine (GpA) dinucleotides by the cytotoxic protein K-sarcin has been studied. Quantitative analysis of the reaction has been performed through reversephase chromatographic (HPLC) separation of the resulting products. The hydrolysis of the 3P-5P phosphodiester bond of these substrates yields the 2P-3P cyclic mononucleotide; this intermediate is converted into the corresponding 3P-monophosphate derivative as the final product of the reaction. The values of the apparent Michaelis constant (KM), kcat and kcat/ KM have also been calculated. The obtained results fit into a twostep mechanism for the enzymatic activity of K-sarcin and allow to consider this protein as a cyclizing RNase. z 1998 Federation of European Biochemical Societies.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationLacadena Javier, Martı́nez del Pozo Alvaro, Lacadena Valle, Martı́nez-Ruiz Antonio, Mancheño José M, Oñaderra Mercedes and Gavilanes José G(1998), The cytotoxin α-sarcin behaves as a cyclizing ribonuclease, FEBS Letters, 424, doi: 10.1016/S0014-5793(98)00137-9
dc.identifier.doi10.1016/s0014-5793(98)00137-9
dc.identifier.issn0014-5793
dc.identifier.issn1873-3468
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103372
dc.journal.titleFEBS Letters
dc.language.isoeng
dc.rights.accessRightsopen access
dc.subject.cdu577.1
dc.subject.keywordDinucleotide;
dc.subject.keywordRibonucleolytic activity;
dc.subject.keywordRibosome-inactivating protein;
dc.subject.keywordRibotoxin
dc.subject.ucmBioquímica (Química)
dc.subject.unesco2302 Bioquímica
dc.titleThe cytotoxin α‐sarcin behaves as a cyclizing ribonuclease
dc.typejournal article
dc.volume.number424
dspace.entity.typePublication
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