Disposable Amperometric Immunosensor for the Detection of Adulteration in Milk through Single or Multiplexed Determination of Bovine, Ovine, or Caprine Immunoglobulins G

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dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorPovedano Muñumel, Eloy
dc.contributor.authorBenedé Pérez, Sara
dc.contributor.authorMata, Luis
dc.contributor.authorGalán-Malo, Patricia
dc.contributor.authorGamella, María
dc.contributor.authorReviejo García, Ángel Julio
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-25T12:47:27Z
dc.date.available2024-01-25T12:47:27Z
dc.date.issued2019
dc.description.abstractThis paper reports the first immunoplatforms for the detection of adulteration in milk with milk or colostrum from other animals. The developed electrochemical bioplatforms allow the reliable determination of immunoglobulins G (IgGs) from cows, sheeps, or goats. They rely on sandwiching each animal species-specific IgGs with selective antibody pairs [unconjugated and conjugated with horseradish peroxidase (HRP)] onto magnetic microbeads (MBs) used as solid supports and amperometric transduction with the hydrogen peroxide/hydroquinone (HQ) system at disposable electrodes. The immunoplatforms allow achieving limits of detection (LODs) of 0.74, 0.82, and 0.66 ng/mL for bovine, ovine, and caprine IgGs, respectively, which are lower than those obtained with conventional enzyme-linked immunosorbent assay (ELISA) methodologies and in 2–5 times shorter time. The bioplatforms were successfully applied to the determination of the individual content of the target IgGs in milk samples of different animals (cow, sheep, and goat) and type (colostrum, raw, and pasteurized), without matrix effect and after just a sample dilution. They were also applied to the detection of adulteration with milks from other animals at levels below than those required by the European legislation (1.0%, v/v). The possibility to detect milk adulteration with colostrum using a strategy based on the measurement of the total content of the three target IgGs in raw milks is also demonstrated. Multiplexing platforms were constructed to be used in routine surveillance of milk. They are able to provide in a single run and in just 30 min relevant information regarding the milk sample including its animal origin, the undergone heat treatment, and whether it was adulterated with milk or colostrum from other species.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationRuiz-Valdepeñas Montiel V, Povedano E, Benedé S, Mata L, Galán-Malo P, Gamella M, Reviejo AJ, Campuzano S, Pingarrón JM. Disposable Amperometric Immunosensor for the Detection of Adulteration in Milk through Single or Multiplexed Determination of Bovine, Ovine, or Caprine Immunoglobulins G. Anal Chem. 2019 Sep 3;91(17):11266-11274. doi: 10.1021/acs.analchem.9b02336. Epub 2019 Aug 20. PMID: 31397563. F
dc.identifier.doi10.1021/acs.analchem.9b02336
dc.identifier.essn1520-6882
dc.identifier.issn0003-2700
dc.identifier.officialurlhttps://doi.org/10.1021/acs.analchem.9b02336
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/31397563/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95439
dc.journal.titleAnalytical Chemistry
dc.language.isoeng
dc.page.final11274
dc.page.initial11266
dc.publisherAmerican Chemical Society
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//CTQ2015-64402-C2-1-R/ES/NUEVAS PLATAFORMAS DE MULTIPLEXADO ELECTROANALITICAS PARA LA DETECCION Y PRONOSTICO DE ENFERMEDADES NEOPLASICAS MEDIANTE BIOPSIAS LIQUIDAS/
dc.relation.projectIDP2018/NMT-4349
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleDisposable Amperometric Immunosensor for the Detection of Adulteration in Milk through Single or Multiplexed Determination of Bovine, Ovine, or Caprine Immunoglobulins G
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number91
dspace.entity.typePublication
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