Oligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target Induced DNA Inter-Strand Ligand Reaction

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dc.contributor.authorMéndez González, Diego
dc.contributor.authorLaurenti, Marco
dc.contributor.authorLatorre, Alfonso
dc.contributor.authorSomoza, Álvaro
dc.contributor.authorVázquez, Ana
dc.contributor.authorNegredo, Ana Isabel
dc.contributor.authorLópez Cabarcos, Enrique
dc.contributor.authorCalderón, Oscar Gómez
dc.contributor.authorMelle Hernández, Sonia
dc.contributor.authorRubio Retama, Benito Jorge
dc.date.accessioned2023-06-17T21:52:46Z
dc.date.available2023-06-17T21:52:46Z
dc.date.issued2017-03-23
dc.descriptionEs una versión postprint del artículo final.
dc.description.abstractWe present a sensor that exploits the phenomenon of upconversion luminescence to detect the presence of specific sequences of small oligonucleotides like miRNAs among others. The sensor is based on NaYF4:Yb,Er@SiO2 nanoparticles functionalized with ssDNA that contain azide groups on the 3' ends. In the presence of a target sequence, inter-strand ligation is possible via click-reaction between one azide of the upconversion probe and a DBCO-ssDNA-biotin probe present in the solution. As result of this specific and selective process, biotin is covalently attached to the surface of the upconversion nanoparticles. The presence of biotin on the surface of the nanoparticles allows their selective capture on a streptavidin-coated support, giving a luminescent signal proportional to the amount of target present in the test samples. With the aim of studying the analytical properties of the sensor, total RNA samples were extracted from healthy mosquitoes and spiked-in with a specific target sequence at different concentrations. The result of these experiments revealed that the sensor was able to detect 10-17 moles (100 fM) of the target sequence in mixtures containing 100 ng of total RNA per well. Similar limit of detection was found for spiked human serum samples, demonstrating its suitability for detecting specific sequences of small oligonucleotides under real conditions. By contrast, under the presence of non-complementary sequences or sequences having mismatches, the luminescent signal was negligible or conspicuously reduced.en
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.departmentSección Deptal. de Óptica (Óptica)
dc.description.facultyFac. de Farmacia
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.sponsorshipBill & Melinda Gates Foundation
dc.description.sponsorshipAsociación Española Contra el Cáncer
dc.description.sponsorshipUniversidad Complutense de Madrid/Banco de Santander
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/42051
dc.identifier.citationMéndez González, D., Laurenti, M., Latorre, A. et al. «Oligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target-Induced DNA Interstrand Ligand Reaction». ACS Applied Materials & Interfaces, vol. 9, n.o 14, abril de 2017, pp. 12272-81. DOI.org (Crossref), https://doi.org/10.1021/acsami.7b00575.
dc.identifier.doi10.1021/acsami.7b00575
dc.identifier.issn1944-8244
dc.identifier.officialurlhttp://dx.doi.org/10.1021/acsami.7b00575
dc.identifier.relatedurlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5391500/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/17708
dc.journal.titleACS applied materials & interfaces
dc.language.isoeng
dc.publisherAmer Chemical Soc.
dc.relation.projectIDMAT2014-55065-R
dc.relation.projectIDFIS2013-41709-P
dc.relation.projectIDSAF2014-56763-R
dc.relation.projectIDOPP1128411
dc.relation.projectIDPR26/16-12B-3
dc.rights.accessRightsopen access
dc.subject.cdu577.113.3
dc.subject.cdu535
dc.subject.keywordOliglonucleotides
dc.subject.keywordSensor
dc.subject.keywordNanoparticles
dc.subject.keywordUpconversion luminiscence
dc.subject.keywordmiRNAs
dc.subject.keywordssDNA
dc.subject.ucmBioquímica (Medicina)
dc.subject.ucmTécnicas de la imagen
dc.titleOligonucleotide Sensor Based on Selective Capture of Upconversion Nanoparticles Triggered by Target Induced DNA Inter-Strand Ligand Reactionen
dc.typejournal article
dspace.entity.typePublication
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