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Effect of cryopreservation on the sperm DNA fragmentation dynamics of the bottlenose dolphin (Tursiops truncatus)

dc.contributor.authorSánchez Calabuig, María Jesús
dc.contributor.authorLópez-Fernández, Carmen
dc.contributor.authorJohnston, Steve D
dc.contributor.authorBlyde, David
dc.contributor.authorCooper, J
dc.contributor.authorHarrison, Keith
dc.contributor.authorde la Fuente, Julio
dc.contributor.authorGosálvez, Jaime
dc.date.accessioned2025-02-05T18:14:34Z
dc.date.available2025-02-05T18:14:34Z
dc.date.issued2015
dc.descriptionSanchez-Calabuig has performed the experiments, the statisticalanalysis and written the manuscript. Dr L opez-Fernandez and DrGos alvez performed the experiment design, the statistical analysis andcorrected the manuscript. Dr Johnston corrected the manuscript andhelped with the experiments. Dr Blyde provided the semen samplesand helped in the experiments. Dr Cooper and Dr Harrison performedsemen samples cryopreservation. Dr de la Fuente has helped in theexperiments in the laboratory
dc.description.abstractSperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species-specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post-thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post-thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax(®)). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES-TRIS-fructose buffer (TTF), an egg-yolk-free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen-thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen-thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1(℗) buffer had higher levels (p < 0.05) of DNA fragmentation after 24- and 48-h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen-thawed samples
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.statuspub
dc.identifier.citationSánchez-Calabuig, López-Fernández, Johnston, Blyde, Cooper, Harrison, de la Fuente, & Gosálvez. (2015). Effect of Cryopreservation on the Sperm DNA Fragmentation Dynamics of the Bottlenose Dolphin (Tursiops truncatus). Reproduction in Domestic Animals, 50(2), 227-235. https://doi.org/10.1111/RDA.12474
dc.identifier.doi10.1111/rda.12474
dc.identifier.essn1439-0531
dc.identifier.issn0936-6768
dc.identifier.officialurlhttps://doi.org/10.1111/rda.12474
dc.identifier.pmid25604784
dc.identifier.urihttps://hdl.handle.net/20.500.14352/117857
dc.issue.number2
dc.journal.titleReproduction in domestic animals
dc.language.isoeng
dc.page.final235
dc.page.initial227
dc.publisherWiley
dc.relation.projectIDBFU-2013-44290-R
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.082.4
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleEffect of cryopreservation on the sperm DNA fragmentation dynamics of the bottlenose dolphin (Tursiops truncatus)
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number50
dspace.entity.typePublication
relation.isAuthorOfPublication8195e871-b27d-4b96-9db7-5076dfd60b94
relation.isAuthorOfPublication.latestForDiscovery8195e871-b27d-4b96-9db7-5076dfd60b94

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