Identification of new allergens in macadamia nut and cross-reactivity with other tree nuts in a Spanish cohort

Simple item page
dc.contributor.authorGutiérrez-Díaz, Gloria
dc.contributor.authorBetancor, Diana
dc.contributor.authorParrón Ballesteros, Jorge
dc.contributor.authorGordo, Rubén G.
dc.contributor.authorCastromil-Benito, Estela S.
dc.contributor.authorHaroum, Eleisa
dc.contributor.authorVázquez de la Torre, María
dc.contributor.authorTurnay Abad, Francisco Javier
dc.contributor.authorVillalba Díaz, María Teresa
dc.contributor.authorCuesta-Herranz, Javier
dc.contributor.authorPastor Vargas, Carlos
dc.date.accessioned2024-07-04T08:25:21Z
dc.date.available2024-07-04T08:25:21Z
dc.date.issued2024-03-26
dc.description.abstractThe consumption of macadamia nuts has increased due to their cardioprotective and antioxidant properties. However, this rise is consistent with an increase in the cases of macadamia nut allergy, leading to severe reactions. Although two macadamia integrifolia allergens (Mac i 1 and Mac i 2) have been identified in Australian and Japanese patients, the allergenic sensitization patterns in Western European populations, particularly in Spain, remain unclear. For this purpose, seven patients with macadamia nut allergy were recruited in Spain. Macadamia nut protein extracts were prepared and, together with hazelnut and walnut extracts, were used in Western blot and inhibition assays. IgE-reactive proteins were identified using MALDI-TOF/TOF mass spectrometry (MS). Immunoblotting assays revealed various IgE-binding proteins in macadamia nut extracts. Mass spectrometry identified three new allergens: an oleosin, a pectin acetylesterase, and an aspartyl protease. Cross-reactivity studies showed that hazelnut extract but not walnut extract inhibited macadamia nut oleosin-specific IgE binding. This suggests that oleosin could be used as marker for macadamia–hazelnut cross-reactivity. The results show an allergenic profile in the Spanish cohort different from that previously detected in Australian and Japanese populations. The distinct sensitization profiles observed highlight the potential influence of dietary habits and environmental factors exposure on allergenicity.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.fundingtypeDescuento UCM
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.statuspub
dc.identifier.citationGutiérrez-Díaz, G.; Betancor, D.; Parrón-Ballesteros, J.; Gordo, R.G.; Castromil-Benito, E.S.; Haroun, E.; Vázquez de la Torre, M.; Turnay, J.; Villalba, M.; Cuesta-Herranz, J.; et al. Identification of New Allergens in Macadamia Nut and Cross-Reactivity with Other Tree Nuts in a Spanish Cohort. Nutrients 2024, 16, 947. https://doi.org/10.3390/nu16070947
dc.identifier.doi10.3390/nu16070947
dc.identifier.officialurlhttps://doi.org/10.3390/nu16070947
dc.identifier.relatedurlhttps://www.mdpi.com/2072-6643/16/7/947
dc.identifier.urihttps://hdl.handle.net/20.500.14352/105601
dc.journal.titleNutrients
dc.language.isoeng
dc.page.initial947
dc.publisherMDPI
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-116692RB-I00/ES/ALERGENOS Y EJE INTESTINO-PULMON: NUEVAS APROXIMACIONES AL DIAGNOSTICO Y TRATAMIENTO DE LA ALERGIA/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu577.1
dc.subject.keywordmacadamia nut
dc.subject.keywordtree nut allergy
dc.subject.keywordoleosin
dc.subject.keywordaspartyl protease
dc.subject.keywordpectin acetylesterase
dc.subject.ucmBioquímica (Química)
dc.subject.unesco2302 Bioquímica
dc.titleIdentification of new allergens in macadamia nut and cross-reactivity with other tree nuts in a Spanish cohort
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number16
dspace.entity.typePublication
relation.isAuthorOfPublicationc8df93e1-6737-4116-8f38-f5814c8e4143
relation.isAuthorOfPublication1cbdc7e2-7732-44c1-99d6-22d32af20103
relation.isAuthorOfPublication8538de4d-b88e-451c-b981-64bcc0bfeede
relation.isAuthorOfPublication25af78c7-0077-4891-a14e-bcd8e51fe408
relation.isAuthorOfPublication.latestForDiscoveryc8df93e1-6737-4116-8f38-f5814c8e4143
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
nutrients-16-00947.pdf
Size:
2.58 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos