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Ghrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (Carassius auratus) via the GHS-R1a: Possible roles of PLC/PKC and AC/PKA intracellular signaling pathways

dc.contributor.authorBlanco Imperiali, Ayelén M.
dc.contributor.authorBertucci, Juan I.
dc.contributor.authorSánchez Bretaño, Aída
dc.contributor.authorDelgado Saavedra, María Jesús
dc.contributor.authorValenciano González, Ana Isabel
dc.contributor.authorUnniappan, Suraj
dc.date.accessioned2023-06-17T22:04:35Z
dc.date.available2023-06-17T22:04:35Z
dc.date.issued2017-02-15
dc.description.abstractGhrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. This study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (Carassius auratus). Immunofluorescence microscopy found colocalization of ghrelin, GHS-R1a and the digestive enzymes sucrase-isomaltase, aminopeptidase A, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. In vitro ghrelin treatment in intestinal and hepatopancreas explant culture led to a concentration- and time-dependent modulation (mainly stimulatory) of most of the digestive enzymes tested. The ghrelin-induced upregulations of digestive enzyme expression were all abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6, and most of them by the phospholipase C inhibitor U73122 or the protein kinase A inhibitor H89. This indicates that ghrelin effects on digestive enzymes are mediated by GHS-R1a, partly by triggering the PLC/PKC and AC/PKA intracellular signaling pathways. These data suggest a role for ghrelin on digestive processes in fish.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipMinisterio de Educación y Ciencia (MEC)
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) (Canada)
dc.description.sponsorshipCanada Foundation for Innovation
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), (Argentina)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44354
dc.identifier.doi10.1016/j.mce.2016.12.027
dc.identifier.issn0303-7207
dc.identifier.officialurlhttps://www.journals.elsevier.com/molecular-and-cellular-endocrinology/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18022
dc.journal.titleMolecular and Cellular Endocrinology
dc.language.isoeng
dc.page.final181
dc.page.initial165
dc.publisherElsevier
dc.relation.projectIDAGL2013-46448-C3-2-R
dc.relation.projectIDDiscovery Grant (341292-2012-RGPIN), and Discovery Accelerator Supplement (429436-2012-RGPAS)
dc.rights.accessRightsrestricted access
dc.subject.cdu591.1
dc.subject.cdu597.2/.5
dc.subject.keywordFish
dc.subject.keywordGhrelin
dc.subject.keywordSucrase-isomaltase
dc.subject.keywordAminopeptidase
dc.subject.keywordTrypsin
dc.subject.keywordLipoprotein lipase
dc.subject.ucmBiología
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmPeces
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2401.13 Fisiología Animal
dc.titleGhrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (Carassius auratus) via the GHS-R1a: Possible roles of PLC/PKC and AC/PKA intracellular signaling pathways
dc.typejournal article
dc.volume.number442
dspace.entity.typePublication
relation.isAuthorOfPublication48071251-a236-4607-ab33-5332c1472628
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication.latestForDiscovery48071251-a236-4607-ab33-5332c1472628

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