Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines

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dc.contributor.authorAlicia Ávalos
dc.contributor.authorMorales Gómez, Paloma
dc.contributor.authorHaza Duaso, Ana Isabel
dc.date.accessioned2024-01-16T16:06:29Z
dc.date.available2024-01-16T16:06:29Z
dc.date.issued2019-06-14
dc.description.abstractMetallic nanoparticles have emerged as an important class of nanomaterials for a wide range of industrial and medical applications. Because of the intensive commercial applications, risk assessment of these nanoparticles is of great importance. In the present study, the human hepatoma and leukemia cells were used to characterize the apoptotic effects of silver nanoparticles (4.7 and 42 nm) and gold nanoparticles (30 nm). Apoptotic cells were identified by chromatin condensation and flow cytometry analysis, using Annexin V/PI, TUNEL and caspase activation assays. Flow cytometry analysis showed that the three metallic nanoparticles induced apoptotic cell death in a concentration and time dependent-manner. Moreover, the three nanoparticles induced activation of caspase-3 and -7 in hepatoma and leukemia cells. Apoptotic effects were stronger after exposure of both cell lines with 4.7 nm silver nanoparticles than those obtained with 42 nm silver and 30 nm gold nanoparticles. In conclusion, silver (4.7 and 42 nm) and gold (30 nm) nanoparticles induced apoptosis in hepatoma and leukemia cells via the caspase dependent pathway. The smaller silver nanoparticles (4.7 nm) had a greater ability to induce apoptosis in both cell lines.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación, Cultura y Deporte (AGL2010-16561)
dc.description.statuspub
dc.identifier.citationAlicia Ávalos, Paloma Morales & Ana Isabel Haza (2018) Manufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines, Toxicological & Environmental Chemistry, 100:5-7, 629-643, DOI: 10.1080/02772248.2018.1545127
dc.identifier.doi10.1080/02772248.2018.1545127
dc.identifier.issn0277-2248
dc.identifier.issn1029-0486
dc.identifier.officialurlhttps://doi.org/10.1080/02772248.2018.1545127
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93433
dc.issue.number5-7
dc.journal.titleToxicological and Environmental Chemistry
dc.language.isoeng
dc.page.final643
dc.page.initial629
dc.publisherTaylor & Francis
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//AGL2010-16561/ES/EVALUACION DE LA SEGURIDAD ALIMENTARIA DE MICROPARTICULAS (COLORANTES) Y NANOPARTICULAS DE ORO Y PLATA EN CELULAS HUMANAS: ESTIMACION DE SUS RIESGOS Y BENEFICIOS POTENCIALES/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu641
dc.subject.keywordApoptosis
dc.subject.keywordcaspases
dc.subject.keywordgold nanoparticles
dc.subject.keywordhuman tumor cells
dc.subject.keywordsilver nanoparticles
dc.subject.ucmTecnología de los alimentos
dc.subject.ucmToxicología (Farmacia)
dc.subject.unesco3309 Tecnología de Los Alimentos
dc.titleManufactured silver and gold nanoparticles-induced apoptosis by caspase-pathway in human cell lines
dc.typejournal article
dc.volume.number100
dspace.entity.typePublication
relation.isAuthorOfPublication80805744-56b4-475b-bb25-b3be6622b96c
relation.isAuthorOfPublication7b71c224-30ca-42fc-be0f-98647103a21f
relation.isAuthorOfPublication.latestForDiscovery7b71c224-30ca-42fc-be0f-98647103a21f
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