Biotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids

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dc.contributor.authorMartínez, Fernando Gabriel
dc.contributor.authorMoreno Martín, Gustavo
dc.contributor.authorPescuma, Micaela
dc.contributor.authorMadrid Albarrán, María Yolanda
dc.contributor.authorMozzi, Fernanda
dc.date.accessioned2024-01-15T09:52:17Z
dc.date.available2024-01-15T09:52:17Z
dc.date.issued2020-06-12
dc.description.abstractSelenium (Se) is an essential micronutrient for the majority of living organisms, and it has been identified as selenocysteine in the active site of several selenoproteins such as glutathione peroxidase, thioredoxin reductase, and deiodinases. Se deficiency in humans is associated with viral infections, thyroid dysfunction, different types of cancer, and aging. In several European countries as well as in Argentina, Se intake is below the recommended dietary Intake (RDI). Some lactic acid bacteria (LAB) can accumulate and bio-transform selenite (toxic) into Se-nanoparticles (SeNPs) and Se-amino acids (non-toxic). The microbial growth, Se metabolite distribution, and the glutathione reductase (involved in selenite reduction) activity of Se-enriched LAB were studied in this work. The ninety-six assayed strains, belonging to the genera Lactococcus, Weissella, Leuconostoc, Lactobacillus, Enterococcus, and Fructobacillus could grow in the presence of 5 ppm sodium selenite. From the total, eight strains could remove more than 80% of the added Se from the culture medium. These bacteria accumulated intracellularly between 1.2 and 2.5 ppm of the added Se, from which F. tropaeoli CRL 2034 contained the highest intracellular amount. These strains produced only the seleno-amino acid SeCys as observed by LC-ICP-MS and confirmed by LC-ESI-MS/MS. The intracellular SeCys concentrations were between 0.015 and 0.880 ppm; Lb. brevis CRL 2051 (0.873 ppm), Lb. plantarum CRL 2030 (0.867 ppm), and F. tropaeoli CRL 2034 (0.625 ppm) were the strains that showed the highest concentrations. Glutathione reductase activity values were higher when the strains were grown in the presence of Se except for the F. tropaeoli CRL 2034 strain, which showed an opposite behavior. The cellular morphology of the strains was not affected by the presence of Se in the culture medium; interestingly, all the strains were able to form spherical SeNPs as determined by transmission electron microscopy (TEM). Only two Enterococcus strains produced the volatile Se compounds dimethyl-diselenide identified by GC-MS. Our results show that Lb. brevis CRL 2051, Lb. plantarum CRL 2030, and F. tropaeoli CRL 2034 could be used for the development of nutraceuticals or as starter cultures for the bio-enrichment of fermented fruit beverages with SeCys and SeNPs.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipSpanish Commission of Science and Technology
dc.description.sponsorshipComunidad of Madrid
dc.description.sponsorshipComplutense University
dc.description.statuspub
dc.identifier.citationMartínez, F. G., Moreno-Martin, G., Pescuma, M., Madrid-Albarrán, Y., & Mozzi, F. (2020). Biotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids. Frontiers in bioengineering and biotechnology, 8, 506.
dc.identifier.doi10.3389/fbioe.2020.00506
dc.identifier.issn2296-4185
dc.identifier.officialurlhttps://www.frontiersin.org/articles/10.3389/fbioe.2020.00506/full
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92993
dc.issue.number506
dc.journal.titleFrontiers in Bioengineering and Biotechnology
dc.language.isoeng
dc.publisherFrontiers
dc.relation.projectIDPIP003-CONICET and FONCyT (PICT2014-0312 andPICT2015-2330)
dc.relation.projectID(ProjectS2018/BAA4393, AVANSECAL-II-CM)
dc.relation.projectIDPre-doctoral grant(CT42/18-CT43/18)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordlactic acid bacteria
dc.subject.keywordselenium metabolism
dc.subject.keywordprobiotic
dc.subject.keywordstarter culture
dc.subject.keywordselenocysteine
dc.subject.keywordmicrobial cell factory
dc.subject.keywordnutraceuticals
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleBiotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number8
dspace.entity.typePublication
relation.isAuthorOfPublicatione2c3f916-865d-4419-b68b-9e52cb1c6cca
relation.isAuthorOfPublication190287de-ed47-4954-86ff-d4f1eddff035
relation.isAuthorOfPublication.latestForDiscoverye2c3f916-865d-4419-b68b-9e52cb1c6cca
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