Detection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar

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dc.contributor.authorBarasona García-Arévalo, José Ángel
dc.contributor.authorBarroso Arévalo, Sandra
dc.contributor.authorRivera Arroyo, Belén
dc.contributor.authorGortázar, Christian
dc.contributor.authorSánchez-Vizcaíno Rodríguez, José Manuel
dc.date.accessioned2023-06-17T09:03:27Z
dc.date.available2023-06-17T09:03:27Z
dc.date.issued2020-03-25
dc.description.abstractThe presence of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex (MTC) is a main concern in wildlife populations such as the Eurasian wild boar (Sus scrofa). Tests detecting antibodies against the MTC are valuable for tuberculosis (TB) monitoring and control and particularly useful in suids. The development of accurate, efficient, and non-invasive new tools to detect exposure to MTC would be highly beneficial for improving disease surveillance. This study aimed to determine if antibodies against MTC could be detected in oral fluid (OF) samples by a new ELISA test (IgG detection) from naturally TB-infected wild boar. For this, individual, paired serum and OF samples were collected from 148 live wild boar in two TB-status areas from Spain and quantitatively used to validate the new ELISA test. Antibodies against MTC were widely detected in OF samples, for which a significant positive correlation (r = 0.83) was found with the validated serology test. OF ELISA sensitivity and specificity were 67.3% and 100%, respectively. The results of this work suggest that OF samples have the potential to be used for MTC diagnosis as a further step in TB surveillance and control in suid populations. Based on our results, further research is warranted and could be performed using non-invasive new tools directly in field conditions to detect exposure to MTC.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. Horizonte 2020
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)/Universidad Complutense
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/65116
dc.identifier.doi10.3390/pathogens9040242
dc.identifier.issn2076-0817
dc.identifier.officialurlhttps://doi.org/10.3390/pathogens9040242
dc.identifier.relatedurlhttps://www.mdpi.com/2076-0817/9/4/242
dc.identifier.urihttps://hdl.handle.net/20.500.14352/8057
dc.issue.number4
dc.journal.titlePathogens
dc.language.isoeng
dc.page.initial242
dc.publisherMDPI
dc.relation.projectIDVACDIVA (862874)
dc.relation.projectIDRTA2015-00033-C02-02
dc.relation.projectIDFJCI-2015-23643; IJCI-2017-33539.
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordanimal tuberculosis
dc.subject.keywordantibody detection
dc.subject.keywordELISA test
dc.subject.keyworddisease surveillance
dc.subject.keywordoral fluid
dc.subject.keywordSus scrofa
dc.subject.ucmAnimales salvajes y exóticos
dc.subject.ucmInmunología veterinaria
dc.subject.unesco3105 Peces y Fauna Silvestre
dc.subject.unesco3109.03 Inmunología
dc.titleDetection of Antibodies against Mycobacterium bovis in Oral Fluid from Eurasian Wild Boar
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
relation.isAuthorOfPublication7ac9cf6b-78dc-4407-85c8-17a3c3652015
relation.isAuthorOfPublication7416bf9e-a5a1-4ef0-8554-e45d89b1c44c
relation.isAuthorOfPublication018c37fd-dcda-4d18-a531-4be5975df1be
relation.isAuthorOfPublicationb078d9ce-ccce-49e2-a4e9-0ce85eca877e
relation.isAuthorOfPublication.latestForDiscovery7ac9cf6b-78dc-4407-85c8-17a3c3652015
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