First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel

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dc.contributor.authorBaldanta Callejo, Sara
dc.contributor.authorArnal, Raquel
dc.contributor.authorBlanco-Rivero, Amaya
dc.contributor.authorGuevara Acosta, Flor Govinda
dc.contributor.authorNavarro Llorens, Juana MarĂ­a
dc.date.accessioned2023-12-20T16:40:21Z
dc.date.available2023-12-20T16:40:21Z
dc.date.issued2023
dc.description.abstractIntroduction: Microorganisms colonize a wide range of natural and artificial environments. Even though most of them are unculturable in laboratory conditions, some ecosystems are ideal niches for bioprospecting extremophiles with unique properties. Up today, there are few reports concerning microbial communities found on solar panels, a widespread, artificial, extreme habitat. Microorganisms found in this habitat belong to drought-, heat- and radiation-adapted genera, including fungi, bacteria, and cyanobacteria. Methods: Here we isolated and identified several cyanobacteria from a solar panel. Then, some strains isolated were characterizated for their resistance to desiccation, UV-C exposition, and their growth on a range of temperature, pH, NaCl concentration or diverse carbon and nitrogen sources. Finally, gene transfer to these isolates was evaluated using several SEVA plasmids with different replicons to assess their potential in biotechnological applications. Results and discussion: This study presents the first identification and characterization of cultivable extremophile cyanobacteria from a solar panel in Valencia, Spain. The isolates are members of the genera Chroococcidiopsis, Leptolyngbya, Myxacorys, and Oculatella all genera with species commonly isolated from deserts and arid regions. Four of the isolates were selected, all of them Chroococcidiopsis, and characterized. Our results showed that all Chroococcidiopsis isolates chosen were resistant up to a year of desiccation, viable after exposition to high doses of UV-C, and capable of being transformed. Our findings revealed that a solar panel is a useful ecological niche in searching for extremophilic cyanobacteria to further study the desiccation and UV-tolerance mechanisms. We conclude that these cyanobacteria can be modified and exploited as candidates for biotechnological purposes, including astrobiology applications.
dc.description.departmentDepto. de BioquĂ­mica y BiologĂ­a Molecular
dc.description.facultyFac. de Ciencias QuĂ­micas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipEuropean Commission
dc.description.statuspub
dc.identifier.citationBaldanta S, Arnal R, Blanco-Rivero A, Guevara G, Navarro Llorens JM. First characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel. Front Microbiol. 2023 Feb 17;14:982422. doi: 10.3389/fmicb.2023.982422. PMID: 36876112; PMCID: PMC9982165.
dc.identifier.doi10.3389/fmicb.2023.982422
dc.identifier.issn1664-302X
dc.identifier.officialurlhttps://doi.org/10.3389/fmicb.2023.982422
dc.identifier.pmid36876112
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91626
dc.journal.titleFrontiers in Microbiology
dc.language.isoeng
dc.page.initial982422
dc.publisherFrontiers
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2015-66960-C3-3-R/ES/APLICACIONES BIOTECNOLOGICAS DE LAS BACTERIAS Y HONGOS RESISTENTES A LA DESECACION Y A LA IRRADIACION/
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//RTI2018-095584-B-C41-42-43-44
dc.relation.projectIDinfo:eu-repo/grantAgreement/Comunidaddemadrid//P2018/BAA-4532
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordChroococcidiopsis
dc.subject.keywordSEVA vectors
dc.subject.keywordUV-C
dc.subject.keywordDesiccation
dc.subject.keywordExtremophile cyanobacteria
dc.subject.keywordSolar panel
dc.subject.ucmCiencias
dc.subject.unesco24 Ciencias de la Vida
dc.titleFirst characterization of cultivable extremophile Chroococcidiopsis isolates from a solar panel
dc.title.alternativePrimera caracterizaciĂ³n de aislamientos cultivables de Chroococcidiopsis extremĂ³fila a partir de un panel solar
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number14
dspace.entity.typePublication
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relation.isAuthorOfPublication41a4d8da-9d33-4a5e-8dab-373eec22fcbe
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relation.isAuthorOfPublication.latestForDiscovery1f61ef61-7cdf-4a15-b67e-e9901f02ec64
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