A further investigation of the leishmaniosis outbreak in Madrid (Spain): low-infectivity phenotype of the Leishmania infantum BOS1FL1 isolate to establish infection in canine cells

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dc.contributor.authorViñals, Luis Miguel
dc.contributor.authorMas Zubiri, Alicia
dc.contributor.authorMartínez Rodrigo, Abel
dc.contributor.authorOrden Gutiérrez, José Antonio
dc.contributor.authorDomínguez Bernal, Gustavo Ramón
dc.contributor.authorCarrión Herrero, Francisco Javier
dc.date.accessioned2024-01-11T14:22:52Z
dc.date.available2024-01-11T14:22:52Z
dc.date.issued2020-12
dc.description.abstractHuman leishmaniosis caused by Leishmania infantum is a zoonotic disease, with dogs as the main reservoir in Mediterranean Basin countries. The largest European outbreak of human leishmaniosis declared in the southwestern Madrid region (Spain) is characterized by unusual epidemiological and clinical features, such as the emergence of new wild reservoirs (hares and rabbits), whereas the seroprevalence, infection, and severity of canine leishmaniosis have not substantially changed since the first studies conducted in Madrid before the outbreak. Previous studies reported that L. infantum isolates from the Madrid leishmaniosis focus displayed elevated virulence in in vivo models of infection and increased infectivity in murine target cells. With the aim of studying whether changes in the host-parasite interaction and virulence profile have developed, we first assessed the behaviour of one circulating isolate of the outbreak, IPER/ES/2012/BOS1FL1 (BOS1FL1), compared to that of a well-characterized strain from canine leishmaniosis, MCAN/ES/1996/BCN150 (BCN150), in terms of infection capacity (percentage of infected cells, representing infectivity, and number of amastigotes per infected cell, representing the intensity of infection) in canine monocytes and macrophages. BCN150 displayed significantly higher infectivity (76.82 ±4.40 vs 38.58 ±2.19; P <0.0001) and intensity of infection (3.64 ±0.13 vs 1.83 ±0.12; P <0.0001) than BOS1FL1 when interacting with canine cells. Our ROS induction results did not differ significantly between the two isolates or with the responses previously described for other L. infantum isolates. Paradoxically, increased resilience to hydrogen peroxide exposure was observed for BOS1FL1 (% viability 40.62 ±5.54 vs 26.37 ±2.93; P =0.039). Finally, we demonstrated that a decreased intracellular load of BOS1FL1 was associated with increased IFN-γ (261.21 ±26.29 vs 69.80 ±9.02; P =0.0151) and decreased IL- 10 production (165.06 ±23.87 vs 264.41 ±30.58; P =0.0002). In this study, we provide the first detailed insight into the differences between the isolate BOS1FL1 from the outbreak in Madrid and the well-characterized strain BCN150 MON-1 obtained from a dog in their response to interacting with canine cells. However, further studies are necessary to shed light on the immune mechanisms resulting in BOS1FL1 exhibiting less virulent behaviour in canine cells than in cells derived from other host species.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUCM-SANTANDER
dc.description.sponsorshipCOMUNIDAD DE MADRID
dc.description.statuspub
dc.identifier.citationMolina-Rueda F, Fernández-González P, Cuesta-Gómez A, Koutsou A, Carratalá-Tejada M, Miangolarra JC. Test-Retest Reliability of a Conventional Gait Model for Registering Joint Angles during Initial Contact and Toe-Off in Healthy Subjects. Int J Environ Res Public Health. 2021 Feb 15;3(18):1343-52
dc.identifier.doi10.1016/j.vetimm.2020.110148
dc.identifier.issn0165-2427
dc.identifier.officialurlhttps://www.sciencedirect.com/journal/veterinary-immunology-and-immunopathology
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92566
dc.journal.titleVeterinary Immunology and Immunopathology
dc.language.isoeng
dc.page.final110148
dc.page.initial110148
dc.publisherElsevier
dc.relation.projectID(PR75/18-21558) UCM-SANTANDER
dc.relation.projectIDPLATESA2-CM (S2018/BAA-4370) COMUNIDAD DE MADRID
dc.rights.accessRightsembargoed access
dc.subject.cdu616.993.161
dc.subject.keywordLeishmania infantum
dc.subject.keywordROS
dc.subject.keywordMonocytes
dc.subject.keywordDogs
dc.subject.keywordVirulence
dc.subject.keywordTropism
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.titleA further investigation of the leishmaniosis outbreak in Madrid (Spain): low-infectivity phenotype of the Leishmania infantum BOS1FL1 isolate to establish infection in canine cells
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number230
dspace.entity.typePublication
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