Changes in histone methylation and acetylation during microspore reprogramming to embryogenesis occur concomitantly with BnHKMT and BnHAT expression and are associated to cell totipotency, proliferation and differentiation in Brassica napus

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dc.contributor.authorRodriguez-Sanz, Héctor
dc.contributor.authorMoreno-Romero, Jordi
dc.contributor.authorSolís González, María Teresa
dc.contributor.authorKöhler, Claudia
dc.contributor.authorRisueño, María
dc.contributor.authorTestillano, Pilar
dc.date.accessioned2024-02-01T12:47:20Z
dc.date.available2024-02-01T12:47:20Z
dc.date.issued2014
dc.description.abstractIn response to stress treatments, microspores can be reprogrammed to become totipotent cells that follow an embryogenic pathway producing haploid and double-haploid embryos which are important biotechnological tools in plant breeding. Recent studies have revealed the involvement of DNA methylation in regulating this process, but no information is available on the role of histone modifications in microspore embryogenesis. Histone modifications are major epigenetic marks controlling gene expression during plant development and in response to environmental changes. Lysine methylation of histones, accomplished by histone lysine methyltransferases (HKMTs), can occur on different lysine residues, with histone H3K9 methylation being mainly associated with transcriptionally silenced regions. In contrast, histone H3 and H4 acetylation is carried out by histone acetyltransferases (HATs) and is associated with actively transcribed genes. In this work, we analyzed 3 different histone epigenetic marks: dimethylation of H3K9 (H3K9me2) and acetylation of H3 and H4 (H3Ac and H4Ac) during microspore embryogenesis in Brassica napus by Western blot and immunofluorescence assays. The expression patterns of histone methyltransferase BnHKMT and histone acetyltransferase BnHAT genes have also been analyzed by qPCR. Our results revealed different spatial and temporal distribution patterns for methylated and acetylated histone variants during microspore embryogenesis and their similarity with the expression profiles of BnHKMT and BnHAT, respectively. The data presented suggest the participation of H3K9me2 and HKMT in embryo cell differentiation and heterochromatinization events, whereas H3Ac, H4Ac, and HAT would be involved in transcriptional activation, totipotency, and proliferation events during cell reprogramming and embryo development.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationRodríguez-Sanz H, Moreno-Romero J, Solís MT, Köhler C, Risueño MC, Testillano PS. Changes in histone methylation and acetylation during microspore reprogramming to embryogenesis occur concomitantly with Bn HKMT and Bn HAT expression and are associated with cell totipotency, proliferation, and differentiation in Brassica napus. Cytogenet Genome Res. 2014;143(1-3):209-18. doi: 10.1159/000365261. Epub 2014 Jul 19. PMID: 25060767.
dc.identifier.doi10.1159/000365261
dc.identifier.officialurlhttps://doi.org/10.1159/000365261
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/25060767/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97689
dc.issue.number1-3
dc.language.isoeng
dc.page.final218
dc.page.initial209
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsmetadata only access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ucmCiencias
dc.subject.unesco31 Ciencias Agrarias
dc.titleChanges in histone methylation and acetylation during microspore reprogramming to embryogenesis occur concomitantly with BnHKMT and BnHAT expression and are associated to cell totipotency, proliferation and differentiation in Brassica napus
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number143
dspace.entity.typePublication
relation.isAuthorOfPublication3da5e099-3087-4d19-8ed0-60a12692e004
relation.isAuthorOfPublication.latestForDiscovery3da5e099-3087-4d19-8ed0-60a12692e004
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