Androgen and Estrogen β Receptor Expression Enhances Efficacy of Antihormonal Treatments in Triple-Negative Breast Cancer Cell Lines

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dc.contributor.authorCrespo, Belén
dc.contributor.authorIllera Del Portal, Juan Carlos
dc.contributor.authorSilván Granado, Gema
dc.contributor.authorLopez-Plaza, Paula
dc.contributor.authorHerrera de la Muela, María
dc.contributor.authorDe La-Puente Yagüe, Miriam
dc.contributor.authorDíaz Del Arco, Cristina
dc.contributor.authorIllera, María José
dc.contributor.authorCáceres Ramos, Sara Cristina
dc.date.accessioned2024-04-25T11:40:57Z
dc.date.available2024-04-25T11:40:57Z
dc.date.issued2024-01-25
dc.descriptionAuthor Contributions Conceptualization, B.C., S.C., P.L.-P., M.H.d.l.M., M.d.l.P.Y., C.D.d.A. and J.C.I.; methodology, B.C., J.C.I., G.S., P.L.-P., M.H.d.l.M., M.d.l.P.Y., C.D.d.A., M.J.I. and S.C.; investigation, B.C., S.C., P.L.-P., M.H.d.l.M., M.d.l.P.Y., C.D.d.A. and J.C.I.; writing—original draft preparation, B.C., S.C. and J.C.I.; writing—review and editing, B.C., S.C., G.S., M.J.I. and J.C.I. All authors have read and agreed to the published version of the manuscript.
dc.description.abstractThe triple-negative breast cancer (TNBC) subtype is characterized by the lack of expression of ERα (estrogen receptor α), PR (progesterone receptor) and no overexpression of HER-2. However, TNBC can express the androgen receptor (AR) or estrogen receptor β (ERβ). Also, TNBC secretes steroid hormones and is influenced by hormonal fluctuations, so the steroid inhibition could exert a beneficial effect in TNBC treatment. The aim of this study was to evaluate the effect of dutasteride, anastrozole and ASP9521 in in vitro processes using human TNBC cell lines. For this, immunofluorescence, sensitivity, proliferation and wound healing assays were performed, and hormone concentrations were studied. Results revealed that all TNBC cell lines expressed AR and ERβ; the ones that expressed them most intensely were more sensitive to antihormonal treatments. All treatments reduced cell viability, highlighting MDA-MB-453 and SUM-159. Indeed, a decrease in androgen levels was observed in these cell lines, which could relate to a reduction in cell viability. In addition, MCF-7 and SUM-159 increased cell migration under treatments, increasing estrogen levels, which could favor cell migration. Thus, antihormonal treatments could be beneficial for TNBC therapies. This study clarifies the importance of steroid hormones in AR and ERβ-positive cell lines of TNBC.
dc.description.departmentDepto. de Fisiología
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencias, Innovación y Universidades
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.identifier.citationCrespo, B., Illera, J. C., Silvan, G., Lopez-Plaza, P., Herrera de la Muela, M., de la Puente Yagüe, M., Diaz Del Arco, C., Illera, M. J., & Caceres, S. (2024). Androgen and Estrogen β Receptor Expression Enhances Efficacy of Antihormonal Treatments in Triple-Negative Breast Cancer Cell Lines. International journal of molecular sciences, 25(3), 1471. https://doi.org/10.3390/ijms25031471
dc.identifier.doi10.3390/ijms25031471
dc.identifier.essn1422-0067
dc.identifier.officialurlhttps://www.mdpi.com/1422-0067/25/3/1471
dc.identifier.pmid38338747
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103499
dc.issue.number3
dc.journal.titleInternational Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial1471
dc.publisherMDPI
dc.relation.projectIDPI22/00314
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu612
dc.subject.keywordandrogen receptor
dc.subject.keywordestrogen receptor beta
dc.subject.keywordsteroid pathway
dc.subject.keywordtriple-negative breast cancer
dc.subject.ucmCiencias
dc.subject.unesco24 Ciencias de la Vida
dc.titleAndrogen and Estrogen β Receptor Expression Enhances Efficacy of Antihormonal Treatments in Triple-Negative Breast Cancer Cell Lines
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number25
dspace.entity.typePublication
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Androgen and Estrogen β Receptor Expression Enhances Efficacy of Antihormonal Treatments in Triple-Negative Breast Cancer Cell Lines. International Journal of Molecular Sciences
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