Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas

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dc.contributor.authorCastro, R
dc.contributor.authorColl, J
dc.contributor.authorJouneau, L
dc.contributor.authorBlanco Gutiérrez, María Del Mar
dc.contributor.authorRodríguez Bertos, Antonio Manuel
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.contributor.authorGibello Prieto, Alicia
dc.date.accessioned2024-01-17T08:39:51Z
dc.date.available2024-01-17T08:39:51Z
dc.date.issued2019-05-02
dc.descriptionAuthors’ contributions AG and MMB designed the study. RC, AG and JC did most of the array experiments and performed the statistical analyses. AG, MMB, JFFG carried out the infection experiments and cared for the fsh. ARB performed the histopathological studies. LJ performed the statistical analyses. The manuscript was written by AG and RC, and critically reviewed by JC, MMB and JFFG who contributed to writing the manuscript. All authors read and approved the final manuscript.
dc.description.abstractLactococcus garvieae is a significant pathogen in aquaculture with a potential zoonotic risk. To begin to characterize the late immune response of trout to lactococcosis, we selected infected individuals showing clinical signs of lactococcosis. At the time lactococcosis clinical signs appeared, infection by L. garvieae induced a robust inflammatory response in the spleen of rainbow trout, which correlated with abundant granulomatous lesions. The response in kidney goes in parallel with that of spleen, and most of the gene regulations are similar in both organs. A correlation existed between the early inflammatory granulomas in spleen (containing macrophages with internalized L. garvieae) and up-regulated gene sets, which defined the presence of macrophages and neutrophils. This is the first analysis of the immune transcriptome of rainbow trout following L. garvieae infection during the initiation of adaptive immune mechanisms and shows a ranscriptome induction of antibody response by both IgM (+) and IgT (+) spleen B cells to respond to systemic infection. These results increase our understanding of lactococcosis and pave the way for future research to improve control measures of lactococcosis on fish farms.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio Español de Economía y Competitividad (AGL 2012-35419 y AGL2014-51773-C3-3-R )
dc.description.sponsorshipComunidad de Madrid (S2013/ABI-2747 (TAVS-CAM))
dc.description.sponsorshipUnión Europea
dc.description.statuspub
dc.identifier.citationCastro R, Coll J, Blanco MDM, Rodriguez-Bertos A, Jouneau L, Fernández-Garayzábal JF, Gibello A. Spleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas. Vet Res. 2019 May 2;50(1):32. doi: 10.1186/s13567-019-0649-8. PMID: 31046823; PMCID: PMC6498643.
dc.identifier.doi10.1186/s13567-019-0649-8
dc.identifier.essn1297-9716
dc.identifier.issn0928-4249
dc.identifier.officialurlhttps://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-019-0649-8
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93517
dc.language.isoeng
dc.page.initial32
dc.publisherSpring Nature
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.09
dc.subject.keywordLactococcus garvieae
dc.subject.keywordGene expression
dc.subject.keywordSpleen
dc.subject.keywordKidney
dc.subject.keywordTrout infection
dc.subject.ucmVeterinaria
dc.subject.unesco31 Ciencias Agrarias
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleSpleen and head kidney differential gene expression patterns in trout infected with Lactococcus garvieae correlate with spleen granulomas
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number50
dspace.entity.typePublication
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