Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling After Myocardial Infarction
dc.contributor.author | Ramos, Isabel | |
dc.contributor.author | Henningsson, Markus | |
dc.contributor.author | Nezafat, Maryam | |
dc.contributor.author | Lavín Plaza, Begoña | |
dc.contributor.author | Lorrio, Silvia | |
dc.contributor.author | Gebhardt, Pierre | |
dc.contributor.author | Protti, Andrea | |
dc.contributor.author | Eykyn, Thomas | |
dc.contributor.author | Andia, Marcelo | |
dc.contributor.author | Floögel, Ulrich | |
dc.contributor.author | Phinikaridou, Alkystis | |
dc.contributor.author | Shah, Ajay | |
dc.contributor.author | Botnar, Reneé | |
dc.date.accessioned | 2024-02-05T15:12:07Z | |
dc.date.available | 2024-02-05T15:12:07Z | |
dc.date.issued | 2018 | |
dc.description.abstract | Background: Optimal healing of the myocardium after myocardial infarction (MI) requires a suitable degree of inflammation and its timely resolution, together with a well-orchestrated deposition and degradation of ECM (extracellular matrix) proteins. Methods and Results: MI and SHAM-operated animals were imaged at 3, 7, 14, and 21 days with 3T magnetic resonance imaging using a 19F/1H surface coil. Mice were injected with 19F-perfluorocarbon nanoparticles to study inflammatory cell recruitment, and with a gadolinium-based elastin-binding contrast agent to evaluate elastin content. 19F magnetic resonance imaging signal colocalized with infarction areas, as confirmed by late gadolinium enhancement, and was highest 7 days post-MI, correlating with macrophage content (MAC-3 immunohistochemistry; ρ=0.89, P<0.0001). 19F quantification with in vivo (magnetic resonance imaging) and ex vivo nuclear magnetic resonance spectroscopy correlated linearly (ρ=0.58, P=0.020). T1 mapping after gadolinium-based elastin-binding contrast agent injection showed increased relaxation rate (R1) in the infarcted regions and was significantly higher at 21 days compared with 7 days post-MI (R1 [s−1]: 21 days=2.8 [interquartile range, 2.69–3.30] versus 7 days=2.3 [interquartile range, 2.12–2.5], P<0.05), which agreed with an increased tropoelastin content (ρ=0.89, P<0.0001). The predictive value of each contrast agent for beneficial remodeling was evaluated in a longitudinal proof-of-principle study. Neither R1 nor 19F at day 7 were significant predictors for beneficial remodeling (P=0.68; P=0.062). However, the combination of both measurements (R1<2.34 Hz and 0.55≤19F≤1.85) resulted in an odds ratio of 30.0 (CI 95%, 1.41–638.15; P=0.029) for favorable post-MI remodeling. Conclusions: Multinuclear 1H/19F magnetic resonance imaging allows the simultaneous assessment of inflammation and elastin remodeling in a murine MI model. The interplay of these biological processes affects cardiac outcome and may have potential for improved diagnosis and personalized treatment. | |
dc.description.department | Depto. de Bioquímica y Biología Molecular | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.status | pub | |
dc.identifier.citation | Ramos, Isabel T., et al. «Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling After Myocardial Infarction». Circulation: Cardiovascular Imaging, vol. 11, n.o 11, noviembre de 2018, p. e007453. https://doi.org/10.1161/CIRCIMAGING.117.007453. | |
dc.identifier.doi | 10.1161/circimaging.117.007453 | |
dc.identifier.essn | 1942-0080 | |
dc.identifier.issn | 1941-9651 | |
dc.identifier.officialurl | https://doi.org/10.1161/CIRCIMAGING.117.007453 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/99050 | |
dc.issue.number | 11 | |
dc.journal.title | Circulation: Cardiovascular Imaging | |
dc.language.iso | eng | |
dc.publisher | American Heart Association | |
dc.rights.accessRights | open access | |
dc.subject.cdu | 577.1 | |
dc.subject.ucm | Bioquímica (Química) | |
dc.subject.unesco | 24 Ciencias de la Vida | |
dc.title | Simultaneous Assessment of Cardiac Inflammation and Extracellular Matrix Remodeling After Myocardial Infarction | |
dc.type | journal article | |
dc.type.hasVersion | AM | |
dc.volume.number | 11 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 1f5cced3-0761-429d-a70e-4881fff2f7a9 | |
relation.isAuthorOfPublication.latestForDiscovery | 1f5cced3-0761-429d-a70e-4881fff2f7a9 |
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