Differential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages

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dc.contributor.authorDíez Orejas, Rosalía María
dc.contributor.authorFeito Castellano, María José
dc.contributor.authorCicuéndez Maroto, Mónica
dc.contributor.authorRojo, J.M.
dc.contributor.authorPortolés Pérez, María Teresa
dc.date.accessioned2024-01-30T17:46:53Z
dc.date.available2024-01-30T17:46:53Z
dc.date.issued2018
dc.description.abstractMacrophages, as effector cells involved in the innate and adaptive immunity, play a key role in the response to nanomaterials as graphene oxide (GO) and in their cellular uptake. The interactions at the interface of GO nanosheets, macrophages and microbial pathogens need to be assessed to determine the possible impairment of the immune system induced by biomedical treatments with this nanomate-rial. Here, we have evaluated by flow cytometry and confocal microscopy the ability of murine peritoneal macrophages to phagocytose the fungal pathogen Candida albicans, alive or heat-killed, after treatment with poly(ethylene glycol-amine)-derivatized GO nanosheets (PEG-GO). After GO treatment, differences in fungal phagocytosis were observed between macrophages that had taken up GO nanosheets (GO+ pop-ulation) and those that had not (GO population). GO treatment increased the ingested alive yeasts in GO macrophages, whereas phagocytosis diminished in the GO+ population. Ingestion of heat-killed yeasts was slightly higher in both GO and GO+ populations when comparing with control macrophages. For the first time, we show that GO uptake by macrophages modulates its phagocytic capability, affecting differentially the subsequent ingestion of either alive or heat-killed yeasts. Enhanced ingestion of heat-killed yeast by GO-treated macrophages suggests a beneficial role of this nanomaterial for the clearance of dead microorganisms during infection.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad(España)
dc.description.sponsorshipFundação para a Ciência e a Tecnologia(Portugal)
dc.description.statuspub
dc.identifier.citationR. Diez-Orejas et al. / Journal of Colloid and Interface Science 512 (2018) 665–673
dc.identifier.doi10.1016/j.jcis.2017.10.104
dc.identifier.officialurlhttps://doi.org/10.1016/j.jcis.2017.10.104
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96728
dc.journal.titleJournal of Colloid and Interface Science
dc.language.isoeng
dc.page.final673
dc.page.initial665
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/MAT2013-43299-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/MAT2016-75611-R AEI/FEDER
dc.relation.projectIDinfo:eu-repo/grantAgreement/SFRH/BPD/101468/2014
dc.relation.projectIDinfo:eu-repo/grantAgreement/PI13/01809
dc.rights.accessRightsrestricted access
dc.subject.keywordGraphene oxide
dc.subject.keywordMacrophage
dc.subject.keywordCandida albicans
dc.subject.keywordPhagocytosis
dc.subject.keywordPhagocytosis
dc.subject.keywordNanomaterial
dc.subject.keywordImmune response
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco23 Química
dc.titleDifferential effects of graphene oxide nanosheets on Candida albicans phagocytosis by murine peritoneal macrophages
dc.typejournal article
dc.volume.number512
dspace.entity.typePublication
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relation.isAuthorOfPublication216318f7-e25a-4850-b122-856eb08b3e2f
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relation.isAuthorOfPublication.latestForDiscoverye9388a5d-dfe6-4703-a755-5e10fc0dc5ff
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