Design of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae

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dc.contributor.authorFeito Hermida, Javier
dc.contributor.authorAraújo, Carlos
dc.contributor.authorArbulu, Sara
dc.contributor.authorContente, Diogo
dc.contributor.authorGómez Sala, Beatriz
dc.contributor.authorDíaz-Formoso, Lara
dc.contributor.authorMuñoz Atienza, Estefanía
dc.contributor.authorBorrero Del Pino, Juan
dc.contributor.authorCintas Izarra, Luis Miguel
dc.contributor.authorHernández Cruza, Pablo Elpidio
dc.date.accessioned2023-06-22T11:25:08Z
dc.date.available2023-06-22T11:25:08Z
dc.date.issued2023-03-02
dc.description.abstractLactococcus garvieae is a main ichthyopathogen in rainbow trout (Oncorhynchus mykiss, Walbaum) farming, although bacteriocinogenic L. garvieae with antimicrobial activity against virulent strains of this species have also been identified. Some of the bacteriocins characterized, such as garvicin A (GarA) and garvicin Q (GarQ), may show potential for the control of the virulent L. garvieae in food, feed and other biotechnological applications. In this study, we report on the design of Lactococcus lactis strains that produce the bacteriocins GarA and/or GarQ, either alone or together with nisin A (NisA) or nisin Z (NisZ). Synthetic genes encoding the signal peptide of the lactococcal protein Usp45 (SPusp45), fused to mature GarA (lgnA) and/or mature GarQ (garQ) and their associated immunity genes (lgnI and garI, respectively), were cloned into the protein expression vectors pMG36c, which contains the P32 constitutive promoter, and pNZ8048c, which contains the inducible PnisA promoter. The transformation of recombinant vectors into lactococcal cells allowed for the production of GarA and/or GarQ by L. lactis subsp. cremoris NZ9000 and their co-production with NisA by Lactococcus lactis subsp. lactis DPC5598 and L. lactis subsp. lactis BB24. The strains L. lactis subsp. cremoris WA2-67 (pJFQI), a producer of GarQ and NisZ, and L. lactis subsp. cremoris WA2-67 (pJFQIAI), a producer of GarA, GarQ and NisZ, demonstrated the highest antimicrobial activity (5.1- to 10.7-fold and 17.3- to 68.2-fold, respectively) against virulent L. garvieae strains.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades
dc.description.sponsorshipUniversidad Complutense de Madrid (UCM)
dc.description.sponsorshipSantander/UCM
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/78752
dc.identifier.doi10.3390/foods12051063
dc.identifier.issn2304-8158
dc.identifier.officialurlhttps://doi.org/10.3390/foods12051063
dc.identifier.relatedurlhttps://www.mdpi.com/2304-8158/12/5/1063
dc.identifier.urihttps://hdl.handle.net/20.500.14352/72398
dc.issue.number5
dc.journal.titleFoods
dc.language.isoeng
dc.page.initial1063
dc.publisherMPDI
dc.relation.projectIDRTI2018-094907-B-I00
dc.relation.projectIDFEI16/54
dc.relation.projectIDPR75/18–21627
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordBacteriocins
dc.subject.keywordGarvicin
dc.subject.keywordnisin
dc.subject.keywordLactococcus garvieae
dc.subject.keywordLactic acid bacteria (LAB)
dc.subject.keywordHeterologous production
dc.subject.keywordLactococcus lactis
dc.subject.keywordProbiotics
dc.subject.keywordParaprobiotics
dc.subject.keywordPostbiotics
dc.subject.ucmMicrobiología (Biología)
dc.subject.unesco2414 Microbiología
dc.titleDesign of Lactococcus lactis Strains Producing Garvicin A and/or Garvicin Q, Either Alone or Together with Nisin A or Nisin Z and High Antimicrobial Activity against Lactococcus garvieae
dc.typejournal article
dc.volume.number12
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery868f9323-f98f-4637-a672-9380b0044828
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