Hemolysis, icterus and lipemia interfere with the determination of two oxidative stress biomarkers in canine serum

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dc.contributor.authorPérez Montero, Blanca
dc.contributor.authorFermín Rodríguez, María Luisa
dc.contributor.authorMiró Corrales, Guadalupe
dc.contributor.authorJuan Ferré, Lucía De
dc.contributor.authorCruz López, Fátima
dc.date.accessioned2024-05-29T14:54:08Z
dc.date.available2024-05-29T14:54:08Z
dc.date.issued2023-09-23
dc.description2023 Acuerdos transformativos CRUE
dc.description.abstractBackground: Oxidative stress has been proven to play a role in numerous human and canine diseases. Among the biomarkers of oxidative stress, Thiobarbituric Acid Reactive Substances (TBARS) and Total Antioxidant Status (TAS) are two of the most widely used. Preanalytical factors are crucial for obtaining accurate results in these assays. Hemolysis, icterus and lipemia (HIL) are common sources of preanalytical errors in the laboratory; however, limited information is available regarding the considerations for canine specimens. Therefore, the objective of this study was to evaluate the potential interferences of HIL in the determination of TBARS and TAS in canine serum. Methods: Solutions of pooled canine serum samples were prepared by adding increasing concentrations of hemolysate, bilirubin and a synthetic lipid emulsion. TBARS and TAS were determined, and biases from the control value caused by the interfering substances were calculated. Results: Hemolysis, icterus and lipemia induced significant interferences on TBARS and TAS, albeit to varying degrees depending on the specific biomarker and interfering substance. TBARS appeared to be more susceptible to interferences in this study. Slight hemolysis, moderate icterus and slight lipemia caused notable deviations in TBARS values, surpassing the acceptable threshold for interference. TAS assay was also affected by HIL, although to a lesser extent compared to TBARS. Significant biases from TAS control value were observed when icterus was moderate, and when hemolysis and lipemia were more pronounced. Conclusions: In light of our results, we conclude that hemolyzed, icteric and lipemic specimens are not suitable for TBARS and TAS determination in canine serum. Our findings hold considerable practical utility, as a simple visual inspection would be sufficient for identifying and excluding such specimens.
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationPerez-Montero, B., Fermin-Rodriguez, M., Miro, G. et al. Hemolysis, icterus and lipemia interfere with the determination of two oxidative stress biomarkers in canine serum. BMC Vet Res 19, 172 (2023). https://doi.org/10.1186/s12917-023-03740-y
dc.identifier.doi10.1186/s12917-023-03740-y
dc.identifier.essn1746-6148
dc.identifier.officialurlhttps://doi.org/10.1186/s12917-023-03740-y
dc.identifier.pmid37741986
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/37741986/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104552
dc.issue.number172
dc.journal.titleBMC Veterinary Research
dc.language.isoeng
dc.publisherSpringer
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordThiobarbituric acid reactive substances
dc.subject.keywordTotal antioxidant status
dc.subject.keywordJaundice
dc.subject.keywordInterference
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleHemolysis, icterus and lipemia interfere with the determination of two oxidative stress biomarkers in canine serum
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number19
dspace.entity.typePublication
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relation.isAuthorOfPublicationa2e597a9-b881-4e99-9faf-b56b882afe90
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relation.isAuthorOfPublication.latestForDiscovery738f3016-240a-40e0-b1ae-e52b7602970e
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Hemolysis, icterus and lipemia interfere with the determination of two oxidative stress biomarkers in canine serum
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