Safety assessment and molecular genetic profiling by pulsed-field gel electrophoresis (PFGE) and PCR-based techniques of Enterococcus faecium strains of food origin

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dc.contributor.authorMuñoz Atienza, Estefanía
dc.contributor.authorDias Araujo, C.
dc.contributor.authorCampo, R.D.
dc.contributor.authorHernández Cruza, Pablo Elpidio
dc.contributor.authorHerranz Sorribes, Carmen
dc.contributor.authorCintas Izarra, Luis Miguel
dc.date.accessioned2024-01-24T13:46:23Z
dc.date.available2024-01-24T13:46:23Z
dc.date.issued2015-08-15
dc.description.abstractEnterococcus faecium is authorized as animal probiotic in the European Union, but this species has emerged as an important cause of nosocomial infections in humans. We investigated the safety of 14 potential probiotic E. faecium strains with antimicrobial activity, previously isolated from food, following the guidance proposed by EFSA. All the enterococci were susceptible to ampicillin, and none of them harbored the genes encoding the enterococcal surface protein (esp), putative glycosyl hydrolase (hylEfm), and insertion sequence IS16. The genetic relatedness of these enterococci was determined by pulsed-field gel electrophoresis (PFGE), random amplified polymorphic DNA (RAPD), enterobacterial repetitive intergenic consensus (ERIC-PCR), and restriction analysis of amplified 16S rDNA (ARDRA). PFGE analysis of SmaI patterns evidenced four subgroups, whereas RAPD and ERIC-PCR analysis gave nine and eight different subgroups, respectively. ERIC-PCR yielded the highest diversity, followed by RAPD and PFGE, while ARDRA achieved the lowest diversity. In conclusion, we demonstrated the absence of well-known enterococcal virulence markers in a collection of E. faecium strains from food, which renders them safe to be used in the food industry or as probiotics in animal production, and that ERIC-PCR is a reliable tool to be used for molecular genetic profiling of potential probiotic enterococci.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Veterinaria
dc.description.refereedFALSE
dc.description.sponsorshipMinisterio de Economía y Competitividad
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipEuropean Social Fund
dc.description.statuspub
dc.identifier.doi10.1016/J.LWT.2015.08.038
dc.identifier.essn0023-6438
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0023643815301328
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95139
dc.journal.titleLWT - Food Science and Technology
dc.language.isoeng
dc.page.final362
dc.page.initial357
dc.publisherElsevier
dc.relation.projectIDAGL2012-34829
dc.relation.projectIDS-2009/AGR-1489
dc.relation.projectIDP2013/ABI-2747
dc.relation.projectID(SFRH/BD/62416/2009
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordEnterococcus faecium
dc.subject.keywordFood origin
dc.subject.keywordSafety assessment
dc.subject.keywordGenetic relatedness
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.titleSafety assessment and molecular genetic profiling by pulsed-field gel electrophoresis (PFGE) and PCR-based techniques of Enterococcus faecium strains of food origin
dc.typejournal article
dc.type.hasVersionAO
dc.volume.number65
dspace.entity.typePublication
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relation.isAuthorOfPublicationbbed512a-7c7b-400a-9248-500dc0877ade
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relation.isAuthorOfPublication.latestForDiscovery22d49290-f994-42ad-b8ce-bfe1f9c114c8
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Safety assessment and molecular genetic profiling by pulsed-field gel electrophoresis (PFGE) and PCR-based techniques of Enterococcus faecium strains of food origin
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