Intercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy

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dc.contributor.authorAhijado Guzmán, Rubén
dc.contributor.authorSánchez Arribas, Natalia
dc.contributor.authorMartínez Negro, María
dc.contributor.authorGonzález Rubio, Guillermo
dc.contributor.authorSantiago-Varela, María
dc.contributor.authorPardo, María
dc.contributor.authorPiñeiro, Antonio
dc.contributor.authorLópez-Montero, Iván
dc.contributor.authorJunquera González, María Elena
dc.contributor.authorGuerrero Martínez, Andrés
dc.date.accessioned2023-06-16T15:21:00Z
dc.date.available2023-06-16T15:21:00Z
dc.date.issued2020-03-11
dc.description.abstractEfficient plasmonic photothermal therapies (PPTTs) using non-harmful pulse laser irradiation at the near-infrared (NIR) are a highly sought goal in nanomedicine. These therapies rely on the use of plasmonic nanostructures to kill cancer cells while minimizing the applied laser power density. Cancer cells have an unsettled capacity to uptake, retain, release, and re-uptake gold nanoparticles, thus offering enormous versatility for research. In this work, we have studied such cell capabilities for nanoparticle trafficking and its impact on the effect of photothermal treatments. As our model system, we chose uveal (eye) melanoma cells, since laser-assisted eye surgery is routinely used to treat glaucoma and cataracts, or vision correction in refractive surgery. As nanostructure, we selected gold nanostars (Au NSs) due to their high photothermal efficiency at the near-infrared (NIR) region of the electromagnetic spectrum. We first investigated the photothermal effect on the basis of the dilution of Au NSs induced by cell division. Using this approach, we obtained high PPTT efficiency after several cell division cycles at an initial low Au NS concentration (pM regime). Subsequently, we evaluated the photothermal effect on account of cell division upon mixing Au NS-loaded and non-loaded cells. Upon such mixing, we observed trafficking of Au NSs between loaded and non-loaded cells, thus achieving effective PPTT after several division cycles under low irradiation conditions (below the maximum permissible exposure threshold of skin). Our study reveals the ability of uveal melanoma cells to release and re-uptake Au NSs that maintain their plasmonic photothermal properties throughout several cell division cycles and re-uptake. This approach may be readily extrapolated to real tissue and even to treat in situ the eye tumor itself. We believe that our method can potentially be used as co-therapy to disperse plasmonic gold nanostructures across affected tissues, thus increasing the effectiveness of classic PPTT.
dc.description.departmentDepto. de Química Física
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/61784
dc.identifier.doi10.3390/nano10030590
dc.identifier.issn2079-4991
dc.identifier.officialurlhttps://www.mdpi.com/2079-4991/10/3/590
dc.identifier.urihttps://hdl.handle.net/20.500.14352/6441
dc.issue.number3
dc.journal.titleNanomaterials
dc.language.isoeng
dc.publisherBMC
dc.relation.projectID(RTI2018-095844-B-I00)
dc.relation.projectIDNANOBIOCARGO (S2018/NMT-4389)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordGold nanostars
dc.subject.keywordnanoparticle endocytosis
dc.subject.keywordnanoparticle exocytosis
dc.subject.keywordfemtosecond pulse laser
dc.subject.keywordplasmonic photothermal therapy
dc.subject.ucmFísica (Química)
dc.subject.ucmBiotecnología
dc.subject.unesco3399 Otras Especialidades Tecnológicas
dc.titleIntercellular Trafficking of Gold Nanostars in Uveal Melanoma Cells for Plasmonic Photothermal Therapy
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublication4af13c82-c400-4bf3-9103-829be26b4d63
relation.isAuthorOfPublicationd590ed92-7a5f-481b-abb0-42ad5d4dbd42
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relation.isAuthorOfPublication.latestForDiscovery4af13c82-c400-4bf3-9103-829be26b4d63
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