Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams

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dc.contributor.authorRebollada Merino, Agustín Miguel
dc.contributor.authorGarcía-Seco Romero, María Teresa
dc.contributor.authorChinchilla Rodríguez, Blanca
dc.contributor.authorPérez Sancho, Marta
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorRodríguez Bertos, Antonio Manuel
dc.date.accessioned2024-05-17T17:30:30Z
dc.date.available2024-05-17T17:30:30Z
dc.date.issued2023-06-07
dc.description2023 Acuerdos transformativos CRUE
dc.description.abstractOvine brucellosis is an infectious disease that causes alterations in the reproductive tract in ram and abortion in ewes. Their negative economic impact in ovine production warrants a thorough understanding the interactions between B. ovis and the host. Here, epididymis lesions of rams infected by B. ovis were histopathologically staged into early and advanced. Expression by immunohistochemistry of Brucella antigens, inflammatory cell markers (CD3, CD79αcy) and cytokines (IFN-γ, TNF-α, TGF-β1) was assessed in both stages. Early lesions were characterized by epithelial changes, interstitial inflammation, and mild fibrosis; whereas advanced lesions displayed caseous granulomas containing numerous macrophages, multinucleated giant cells, lymphocytes, and plasma cells. Expression of Brucella antigens were observed in both stages. The cellular response in B. ovis lesions were predominantly of T-cells (CD3+) whereas low numbers of B-cells and plasma cells (CD79αcy+) were present in both early and advanced lesions. IFN-γ was expressed by lymphocytes in early lesions suggesting that the adaptive immune response against B. ovis is initiated by Th1 cells, this response was also preserved in advanced stages. Expression of TNF-α was observed in neutrophils of epithelial microabscesses and intraepithelial T-cells of early lesions suggesting a promotion of neutrophil phagocytosis triggered by TNF-α. On the other hand, advanced lesions showed a reduction of TNF-α expression which may permit B. ovis persistence in granulomas. Lastly, TGF-β1 expression (fibroblast, macrophages and less in lymphocytes) were increased with time, suggesting that B. ovis promotes TGF-β1 secretion promoting chronicity of the lesions.
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.statuspub
dc.identifier.citationRebollada-Merino, A., García-Seco, T., Chinchilla, B., Pérez-Sancho, M., Domínguez, L., & Rodríguez-Bertos, A. (2023). Immunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams. Veterinary immunology and immunopathology, 261, 110621. https://doi.org/10.1016/j.vetimm.2023.110621
dc.identifier.doi10.1016/j.vetimm.2023.110621
dc.identifier.essn0165-2427
dc.identifier.officialurlhttps://doi.org/10.1016/j.vetimm.2023.110621
dc.identifier.pmid37348444
dc.identifier.relatedurlhttps://www.sciencedirect.com/journal/veterinary-immunology-and-immunopathology
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104186
dc.issue.number110621
dc.journal.titleVeterinary Immunology and Immunopathology
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098658-B-C22/ES/EL SISTEMA DE DOS COMPONENTES WZM%2FWZT DE BRUCELLA: ESTUDIOS MOLECULARES, INTERACCIONES PATOGENO-HOSPEDADOR EN OVINO Y APLICACIONES EN B. SUIS/
dc.relation.projectIDPRE2019-087439
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.3.09
dc.subject.keywordBrucella ovis
dc.subject.keywordHistopathology
dc.subject.keywordImmune response
dc.subject.keywordEpididymitis
dc.subject.keywordLesions
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleImmunopathology of early and advanced epididymis lesions caused by Brucella ovis in rams
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number261
dspace.entity.typePublication
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