Silicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep.

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dc.contributor.authorCasarrubios Molina, Laura
dc.contributor.authorGómez Cerezo, María Natividad
dc.contributor.authorSánchez Salcedo, Sandra
dc.contributor.authorFeito Castellano, María José
dc.contributor.authorSerrano, M.C.
dc.contributor.authorSaiz-Pardo, M.
dc.contributor.authorOrtega Menor, Lorena
dc.contributor.authorDe Pablo, D.
dc.contributor.authorDíaz-Güemes, I.
dc.contributor.authorFernández-Tomé, E.
dc.contributor.authorEnciso, S
dc.contributor.authorPortolés Pérez, María Teresa
dc.contributor.authorSanchez-Margallo, F.M
dc.contributor.authorArcos Navarrete, Daniel
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.contributor.authorSanchez-Margallo, F. M.
dc.date.accessioned2023-06-16T15:15:22Z
dc.date.available2023-06-16T15:15:22Z
dc.date.issued2019-10-31
dc.descriptionRESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete) ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí) RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez) ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)
dc.description.abstractSilicon-substituted hydroxyapatite (SiHA) macroporous scaffolds have been prepared by robocasting. In order to optimize their bone regeneration properties, we have manufactured these scaffolds presenting different microstructures: nanocrystalline and crystalline. Moreover, their surfaces have been decorated with vascular endothelial growth factor (VEGF) to evaluate the potential coupling between vascularization and bone regeneration. In vitro cell culture tests evidence that nanocrystalline SiHA hinders pre-osteblast proliferation, whereas the presence of VEGF enhances the biological functions of both endothelial cells and pre-osteoblasts. The bone regeneration capability has been evaluated using an osteoporotic sheep model. In vivo observations strongly correlate with in vitro cell culture tests. Those scaffolds made of nanocrystalline SiHA were colonized by fibrous tissue, promoted inflammatory response and forested osteoclast recruitment. These observations discard nanocystalline SiHA as a suitable material for bone regeneration purposes. On the contrary, those scaffolds made of crystalline SiHA and decorated with VEGF exhibited bone regeneration properties, with high ossification degree, thicker trabeculae and higher presence of osteoblasts and blood vessels. Considering these results, macroporous scaffolds made of SiHA and decorated with VEGF are suitable bone grafts for regeneration purposes, even in adverse pathological scenarios such as osteoporosis.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. H2020
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statusinpress
dc.eprint.idhttps://eprints.ucm.es/id/eprint/57824
dc.identifier.doi10.1016/j.actbio.2019.10.033
dc.identifier.issn1742-7061
dc.identifier.officialurlhttps://www.journals.elsevier.com/acta-biomaterialia
dc.identifier.relatedurlhttp://www.ucm.es/valletregigroup
dc.identifier.urihttps://hdl.handle.net/20.500.14352/5967
dc.journal.titleActa Biomaterialia
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDVERDI (694160)
dc.relation.projectIDMAT2016-75611-R
dc.rights.accessRightsopen access
dc.subject.cdu546
dc.subject.cdu615.46
dc.subject.cdu66
dc.subject.keywordSilicon substituted hydroxyapatite
dc.subject.keywordMacroporous scaffold
dc.subject.keywordVEGF
dc.subject.keywordOsteoporosis
dc.subject.keywordIn vivo test
dc.subject.ucmMateriales
dc.subject.ucmQuímica inorgánica (Farmacia)
dc.subject.unesco3312 Tecnología de Materiales
dc.titleSilicon substituted hydroxyapatite/VEGF scaffolds stimulate bone regeneration in osteoporotic sheep.
dc.typejournal article
dspace.entity.typePublication
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