Fractal analysis to assess the differentiation state of oligodendroglia in culture

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dc.contributor.authorPascual-Guerra, J.
dc.contributor.authorTorres-Rico, M.
dc.contributor.authorPaíno, C.L.
dc.contributor.authorRodríguez Navarro, José Antonio
dc.contributor.authorGarcía, A.G.
dc.date.accessioned2025-02-18T16:17:00Z
dc.date.available2025-02-18T16:17:00Z
dc.date.issued2024-12-07
dc.descriptionThis work was supported by ISCIII [CP13–00234, CP19–0010]; MINECO [SAF2013–45570-R, SAF2016–78666R], FEDER; Project NDG09/014 of Agencia Laín Entralgo; Project VEXEM; IRYCIS; European Social Fund through Programa Operativo de Empleo Juvenil and Iniciativa de Empleo Juvenil (YEI) of Comunidad de Madrid [PEJ16/MED/AI-1153, PEJD-2018-PRE/SAL-8532].
dc.description.abstractBackground Oligodendroglial development is accompanied by increased cell complexity. A simple and cost-effective evaluation of the pro-myelinating activity of different drugs and/or treatments would be of great interest. In cultured oligodendroglia, an evaluation of the pro-myelinating activity of different drugs and/or treatments can be achieved through fractal analysis, which allows measuring cell complexity. New Method Fractal dimension was assessed in two O4+ cell types (neural stem cell-derived and lineage-converted adipose tissue mesenchymal cells) under proliferating or differentiating conditions. Comparison with Existing Methods This analysis, which was originally developed to analyze microglia, assigns a quantitative value (fractal dimension) to cellular profiles, obtaining higher coefficients as cells increase in size and arborizations instead of mRNA or protein quantification of mature oligodendroglial markers, such as MBP, MAG, O1 or PLP1/DM20. Results This article describes a methodology to perform fractal analysis in immunofluorescent images of O4-positive (O4+) oligodendroglia using the FracLac plugin of ImageJ software. Pro-myelinating drug Benztropine-treated O4+ cells exhibit higher fractal dimension than control group. Conclusions The results demonstrated the effectiveness and sensitivity of the fractal dimension coefficient provided by FracLac software to assess the effects of treatments on oligodendroglial differentiation
dc.description.departmentDepto. de Biología Celular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipAgencia Laín Entralgo
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationPascual-Guerra, J., Torres-Rico, M., Paíno, C. L., Rodríguez-Navarro, J. A., & García, A. G. (2025). Fractal analysis to assess the differentiation state of oligodendroglia in culture. Journal of Neuroscience Methods, 415, 110336. https://doi.org/10.1016/j.jneumeth.2024.110336
dc.identifier.doi10.1016/j.jneumeth.2024.110336
dc.identifier.essn1872-678X
dc.identifier.issn0165-0270
dc.identifier.officialurlhttps://doi.org/10.1016/j.jneumeth.2024.110336
dc.identifier.urihttps://hdl.handle.net/20.500.14352/118165
dc.issue.number110336
dc.journal.titleJournal of Neuroscience Methods
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//SAF2013-45570-R/ES/NUEVAS MODIFICACIONES LIPIDICAS IMPLICADAS EN AUTOPHAGIA. PAPEL EN LA ENFARMEDAD DE PARKINSON Y EL ENVEJECIMIENTO/
dc.rights.accessRightsrestricted access
dc.subject.cdu519.6
dc.subject.cdu576.32
dc.subject.cdu611.81
dc.subject.cdu612.82
dc.subject.keywordCell complexity
dc.subject.keyworddeveloping oligodendroglia
dc.subject.keywordfractal dimension
dc.subject.keywordFrac
dc.subject.keywordLacremyelination
dc.subject.ucmBiología celular (Biología)
dc.subject.ucmNeurociencias (Medicina)
dc.subject.unesco2490 Neurociencias
dc.subject.unesco2407.05 Cultivo de Tejidos
dc.titleFractal analysis to assess the differentiation state of oligodendroglia in culture
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number415
dspace.entity.typePublication
relation.isAuthorOfPublicationde5964e5-0670-496b-baea-3746ef9d6c9c
relation.isAuthorOfPublication.latestForDiscoveryde5964e5-0670-496b-baea-3746ef9d6c9c

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