Publication:
Invasive Species as Hosts of Zoonotic Infections: The Case of American Mink (Neovison vison) and Leishmania infantum

dc.contributor.authorAzami Conesa, Iris
dc.contributor.authorSansano Maestre, José
dc.contributor.authorMartínez Díaz, Rafael Alberto
dc.contributor.authorGómez Muñoz, María Teresa
dc.date.accessioned2023-06-16T14:26:23Z
dc.date.available2023-06-16T14:26:23Z
dc.date.issued2021-07-18
dc.description.abstractLeishmania infantum produces an endemic disease in the Mediterranean Basin that affects humans and domestic and wild mammals, which can act as reservoir or minor host. In this study, we analyzed the presence of the parasite in wild American minks, an invasive species in Spain. We screened for L. infantum DNA by PCR using five primer pairs: Two targeting kinetoplast DNA (kDNA), and the rest targeting the ITS1 region, the small subunit of ribosomal RNA (SSU) and a repetitive sequence (Repeat region). The detection limit was determined for each method using a strain of L. infantum and a bone marrow sample from an infected dog. PCR approaches employing the Repeat region and kDNA (RV1/RV2 primers) showed higher sensitivity than the other PCR methods when control samples were employed. However, only PCR of the Repeat region and nested PCR of SSU (LnSSU) detected the parasite in the samples, while the other three were unable to do so. The majority of the analyzed animals (90.1%) tested positive. American mink may act as an incidental host of the disease for other mammals and should be further investigated, not only for their negative impact on the local fauna, but also as carriers of zoonotic diseases.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUniversity Complutense of Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77434
dc.identifier.doi10.3390/microorganisms9071531
dc.identifier.issn2076-2607
dc.identifier.officialurlhttps://doi.org/10.3390/microorganisms9071531
dc.identifier.relatedurlhttps://www.mdpi.com/2076-2607/9/7/1531
dc.identifier.urihttps://hdl.handle.net/20.500.14352/5035
dc.issue.number7
dc.journal.titleMicroorganisms
dc.language.isoeng
dc.page.initial1531
dc.publisherMPDI
dc.relation.projectIDGR29/20 UCM
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordAmerican mink
dc.subject.keywordITS
dc.subject.keywordkDNA
dc.subject.keywordLeishmaniasis
dc.subject.keywordOne health
dc.subject.keywordHosts
dc.subject.keywordRepeat region
dc.subject.keywordSSUrRNA
dc.subject.keywordWild carnivore
dc.subject.keywordZoonosis
dc.subject.ucmInmunología veterinaria
dc.subject.ucmSanidad animal
dc.subject.unesco3109.03 Inmunología
dc.titleInvasive Species as Hosts of Zoonotic Infections: The Case of American Mink (Neovison vison) and Leishmania infantum
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
relation.isAuthorOfPublication84c594c9-7582-49b2-9dae-77431b96db3a
relation.isAuthorOfPublication1c164bfa-b7f9-4f89-86e5-0d00deae6345
relation.isAuthorOfPublication.latestForDiscovery84c594c9-7582-49b2-9dae-77431b96db3a
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
microorganisms-09-01531.pdf
Size:
661.17 KB
Format:
Adobe Portable Document Format
Collections