Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers

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dc.contributor.authorPedrero Muñoz, María
dc.contributor.authorManuel de Villena Rueda, Francisco Javier
dc.contributor.authorMuñoz San Martín, Cristina
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorGarranzo Asensio, María
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-18T00:02:05Z
dc.date.available2023-06-18T00:02:05Z
dc.date.issued2016-11-21
dc.description.abstractAn amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/66143
dc.identifier.doi10.3390/bios6040056
dc.identifier.issn2079-6374
dc.identifier.officialurlhttps://doi.org/10.3390/bios6040056
dc.identifier.relatedurlhttps://www.mdpi.com/2079-6374/6/4/56
dc.identifier.urihttps://hdl.handle.net/20.500.14352/19176
dc.issue.number4
dc.journal.titleBiosensors
dc.language.isoeng
dc.page.initial56
dc.publisherMDPI
dc.relation.projectIDCTQ2015-64402-C2-1-R
dc.relation.projectIDNANOAVANSENS-CM (S2013/MT–3029)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordhuman p53
dc.subject.keywordmagnetic microcarriers
dc.subject.keywordscreen-printed electrodes
dc.subject.keywordamperometric immunosensor
dc.subject.keywordcell lysates
dc.subject.ucmBioquímica (Química)
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleDisposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers
dc.typejournal article
dc.volume.number6
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery422f8844-54d1-4615-98e8-ef5a0c7e3519
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