Bile-induced promoters for gene expression in Lactobacillus strains

Simple item page
dc.contributor.authorJosé Alberto Martínez Fernández
dc.contributor.authorÁngela Peirotén
dc.contributor.authorJosé María Landete
dc.contributor.authorBravo Vázquez, Daniel Antonio
dc.contributor.authorArqués Orobón, Juan Luis
dc.date.accessioned2024-02-01T00:19:43Z
dc.date.available2024-02-01T00:19:43Z
dc.date.issued2019-03-18
dc.description.abstractBioengineering of probiotics allows the improvement of their beneficial characteristics. In this work, we develop a molecular tool that would allow the activation of desirable traits in probiotics once they reach the intestine. The activity of upstream regions of bile-inducible genes of Lactobacillus casei BL23 and Lactobacillus plantarum WCFS1 was analyzed using plasmids encoding an anaerobic fluorescent protein as reporter. The promoter P16090 from Lb. casei BL23 was selected and its bile induction confirmed in Lb. casei BL23, Lb. plantarum WCFS1, and in Lactobacillus rhamnosus and Lactobacillus reuteri strains. However, the induction did not occur in Lactococcus lactis MG1363 or Bifidobacterium strains. Studies with different bile compounds revealed the importance of cholic acid in the bile induction process. Induction of fluorescence was also confirmed for transformed Lb. casei BL23 under simulated colonic conditions and in the presence of intestinal microbiota. The developed vector, pNZ:16090-aFP, constitutes a promising tool suitable for the expression of genes of interest under intestinal conditions in probiotic strains of the species Lb. casei, Lb. plantarum, Lb. rhamnosus, and Lb. reuteri.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationAppl Microbiol Biotechnol . 2019 May;103(9):3819-3827
dc.identifier.doi10.1007/s00253-019-09743-w
dc.identifier.issn0175-7598
dc.identifier.officialurlhttps://link.springer.com/article/10.1007/s00253-019-09743-w
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97438
dc.issue.number9
dc.journal.titleApplied genetics and molecular biotechnology
dc.language.isoeng
dc.page.final3827
dc.page.initial3819
dc.publisherSpriger Link
dc.rights.accessRightsrestricted access
dc.subject.keywordLactobacillus . Gene expression . Probiotic . Promoter . Bile induction
dc.subject.ucmCiencias
dc.subject.unesco24 Ciencias de la Vida
dc.titleBile-induced promoters for gene expression in Lactobacillus strains
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number103
dspace.entity.typePublication
relation.isAuthorOfPublicationc2079d3a-9509-477c-9aba-1c278053fffe
relation.isAuthorOfPublication5249d481-622b-45d8-b043-187129b69bb1
relation.isAuthorOfPublication.latestForDiscoveryc2079d3a-9509-477c-9aba-1c278053fffe
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Letters in Applied Microbiology 2019.pdf
Size:
813.87 KB
Format:
Adobe Portable Document Format
Download
Collections
Artículos