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Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform

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dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorPedrero Muñoz, María
dc.contributor.authorFernández Aceñero, María Jesús
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-25T12:53:18Z
dc.date.available2024-01-25T12:53:18Z
dc.date.issued2016
dc.description.abstractA sensitive and rapid method for the determination of the clinically relevant biomarker human endoglin (CD105) in serum samples is presented, involving a magneto-actuated immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs). Micro-sized magnetic particles were modified with a specific antibody to selectively capture the target protein which was further sandwiched with a secondary HRP-labeled antibody. The immunocomplexes attached to the magnetic carriers were amperometrically detected at SPCEs using the hydroquinone (HQ)/H2O2/HRP system. The magneto-actuated immunosensing platform was able to detect 5 pmoles of endoglin (in 25μL of sample, 0.2μM) in 30min providing statistically similar results to those obtained using a commercial ELISA kit for the determination of endogenous content of endoglin in human serum samples.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.identifier.citationTorrente-Rodríguez RM, Campuzano S, Ruiz-Valdepeñas-Montiel V, Pedrero M, Fernández-Aceñero MJ, Barderas R, Pingarrón JM. Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform. J Pharm Biomed Anal. 2016 Sep 10;129:288-293. doi: 10.1016/j.jpba.2016.07.020. Epub 2016 Jul 15. PMID: 27448312.
dc.identifier.doi10.1016/j.jpba.2016.07.020
dc.identifier.issn0731-7085
dc.identifier.officialurlhttps://doi.org/10.1016/j.jpba.2016.07.020
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95444
dc.journal.titleJournal of Pharmaceutical and Biomedical Analysis
dc.language.isoeng
dc.page.final293
dc.page.initial288
dc.publisherElsevier
dc.relation.projectIDCTQ2015-64402-C2-1-R
dc.relation.projectIDS2013/MT–3029
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordAmperometric immunosensor
dc.subject.keywordCD105
dc.subject.keywordMagnetic beads
dc.subject.keywordScreen-printed electrodes
dc.subject.keywordAmperometric immunosensor
dc.subject.keywordSerum samples
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleRapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number129
dspace.entity.typePublication
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