Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states

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dc.contributor.authorRomero-Lastra, Patricia
dc.contributor.authorSánchez Beltrán, María Del Carmen
dc.contributor.authorRibeiro-Vidal, Honorato
dc.contributor.authorLlama Palacios, María Arantxazu
dc.contributor.authorFiguero Ruiz, Elena
dc.contributor.authorHerrera González, David
dc.contributor.authorSanz Alonso, Mariano
dc.date.accessioned2024-01-22T19:01:22Z
dc.date.available2024-01-22T19:01:22Z
dc.date.issued2017
dc.description.abstractBackground and objective Porphyromonas gingivalis is a keystone pathogen in the onset and progression of periodontitis. Its pathogenicity has been related to its presence and survival within the subgingival biofilm. The aim of the present study was to compare the genome-wide transcription activities of P. gingivalis in biofilm and in planktonic growth, using microarray technology. Material and methods P. gingivalis ATCC 33277 was incubated in multi-well culture plates at 37˚C for 96 hours under anaerobic conditions using an in vitro static model to develop both the planktonic and biofilm states (the latter over sterile ceramic calcium hydroxyapatite discs). The biofilm development was monitored by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). After incubation, the bacterial cells were harvested and total RNA was extracted and purified. Three biological replicates for each cell state were independently hybridized for transcriptomic comparisons. A linear model was used for determining differentially expressed genes and reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to confirm differential expression. The filtering criteria of ±2 change in gene expression and significance p-values of <0.05 were selected. Results A total of 92 out of 1,909 genes (4.8%) were differentially expressed by P. gingivalis growing in biofilm compared to planktonic. The 54 up-regulated genes in biofilm growth were mainly related to cell envelope, transport, and binding or outer membranes proteins. Thirty-eight showed decreased expression, mainly genes related to transposases or oxidative stress. Conclusion The adaptive response of P. gingivalis in biofilm growth demonstrated a differential gene expression.
dc.description.departmentDepto. de Especialidades Clínicas Odontológicas
dc.description.facultyFac. de Odontología
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationRomero-Lastra P, Sánchez MC, Ribeiro-Vidal H, Llama-Palacios A, Figuero E, Herrera D, Sanz M. Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states. PLoS One. 2017 Apr 3;12(4):e0174669.
dc.identifier.doi10.1371/journal.pone.0174669
dc.identifier.issn1932-6203
dc.identifier.officialurlhttps://doi.org/10.1371/journal.pone.0174669
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94547
dc.issue.number4
dc.journal.titlePlos one
dc.language.isoeng
dc.page.initiale0174669
dc.publisherPublic Library of Science
dc.rights.accessRightsopen access
dc.subject.cdu616.314-002-08
dc.subject.cdu616.31-022
dc.subject.ucmPeriodoncia
dc.subject.ucmMicrobiología médica
dc.subject.unesco3213.13 Ortodoncia-Estomatología
dc.subject.unesco3201.03 Microbiología Clínica
dc.titleComparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number12
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery27fce85c-05b6-4420-b0a4-22ef8510f4e2
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