Characterization of the Neospora caninum NcROP40 and NcROP2Fam-1 rhoptry proteins during the tachyzoite lytic cycle

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dc.contributor.authorRegidor-Cerrillo, Javier
dc.contributor.authorJiménez-Ruiz, Elena
dc.contributor.authorMarugán-Hernández, Virginia
dc.contributor.authorHemphill, Andrew
dc.contributor.authorPastor Fernández, Iván
dc.contributor.authorÁlvarez García, Gema
dc.contributor.authorOrtega Mora, Luis Miguel
dc.date.accessioned2024-02-08T19:08:11Z
dc.date.available2024-02-08T19:08:11Z
dc.date.issued2015-11-02
dc.description.abstractVirulence factors from the ROP2-family have been extensively studied in Toxoplasma gondii, but in the closely related Neospora caninum only NcROP2Fam-1 has been partially characterized to date. NcROP40 is a member of this family and was found to be more abundantly expressed in virulent isolates. Both NcROP2Fam-1 and NcROP40 were evaluated as vaccine candidates and exerted a synergistic effect in terms of protection against vertical transmission in mouse models, which suggests that they may be relevant for parasite pathogenicity. NcROP40 is localized in the rhoptry bulbs of tachyzoites and bradyzoites, but in contrast to NcROP2Fam-1, the protein does not associate with the parasitophorous vacuole membrane due to the lack of arginine-rich amphipathic helix in its sequence. Similarly to NcROP2Fam-1, NcROP40 mRNA levels are highly increased during tachyzoite egress and invasion. However, NcROP40 up-regulation does not appear to be linked to the mechanisms triggering egress. In contrast to NcROP2Fam-1, phosphorylation of NcROP40 was not observed during egress. Besides, NcROP40 secretion into the host cell was not successfully detected by immunofluorescence techniques. These findings indicate that NcROP40 and NcROP2Fam-1 carry out different functions, and highlight the need to elucidate the role of NcROP40 within the lytic cycle and to explain its relative abundance in tachyzoites.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMINECO
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Educación, Cultura y Deporte
dc.description.sponsorshipSwiss National Science Foundation
dc.description.statuspub
dc.identifier.doi10.1017/S0031182015001511
dc.identifier.essn1469-8161
dc.identifier.issn0031-1820
dc.identifier.officialurlhttps://www.cambridge.org/core/journals/parasitology/article/characterization-of-the-neospora-caninum-ncrop40-and-ncrop2fam1-rhoptry-proteins-during-the-tachyzoite-lytic-cycle/B53D6E793C8DB5C0B96C1CD1E6E5DDBC
dc.identifier.pmid26521890
dc.identifier.relatedurlhttps://boris.unibe.ch/82065/1/Pastor-Fernandez%20et%20al.%2C%202016.pdf
dc.identifier.relatedurlhttps://eprints.gla.ac.uk/129577/1/129577.pdf
dc.identifier.urihttps://hdl.handle.net/20.500.14352/100625
dc.issue.number1
dc.journal.titleParasitology
dc.language.isoeng
dc.page.final113
dc.page.initial97
dc.publisherCambridge University Press
dc.relation.projectIDAGL2010-22191/GAN
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2013-44694-R/ES/VIRULENCIA EN LA NEOSPOROSIS BOVINA: ESTUDIOS IN VIVO E IN VITRO DE LA INTERACCION PARASITO-HOSPEDADOR CON AISLADOS DE BAJA%2FALTA VIRULENCIA/
dc.relation.projectIDS2013/ABI2906
dc.relation.projectIDAP2009- 0354
dc.relation.projectID310030 146162
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu61
dc.subject.keywordNeospora caninum
dc.subject.keywordNcROP40
dc.subject.keywordNcROP2Fam-1
dc.subject.keywordCharacterization
dc.subject.keywordIn silico analysis
dc.subject.keywordLytic cycle of tachyzoites
dc.subject.keywordImmunolocalization
dc.subject.keywordSecretion assays
dc.subject.keywordmRNA expression profile
dc.subject.keywordProtein phosphorylation
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleCharacterization of the Neospora caninum NcROP40 and NcROP2Fam-1 rhoptry proteins during the tachyzoite lytic cycle
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number143
dspace.entity.typePublication
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relation.isAuthorOfPublication7a652f61-dd56-4eaa-b148-f4355647bad6
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Characterization of the Neospora caninum NcROP40 and NcROP2Fam-1 rhoptry proteins during the tachyzoite lytic cycle
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