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Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics

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dc.contributor.authorSingh, Mandeep
dc.contributor.authorGuzmán Aránguez, Ana Isabel
dc.contributor.authorHussain, Afzal
dc.contributor.authorSrinivas, Cheerneni S.
dc.contributor.authorKaur, Indu P.
dc.date.accessioned2023-06-17T13:22:07Z
dc.date.available2023-06-17T13:22:07Z
dc.date.issued2019-01-29
dc.description.abstractAim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system.
dc.description.departmentUnidad Docente de Bioquímica y Biología Molecular
dc.description.facultyFac. de Óptica y Optometría
dc.description.refereedTRUE
dc.description.sponsorshipDepartment of Biotechnology (DBT), New Delhi, India
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/55110
dc.identifier.doi10.2217/nnm-2018-0278
dc.identifier.issn1743-5889
dc.identifier.officialurlhttps://doi.org/10.2217/nnm-2018-0278
dc.identifier.relatedurlhttps://www.futuremedicine.com/doi/abs/10.2217/nnm-2018-0278?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=nnm
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13263
dc.issue.number4
dc.journal.titleNanomedicine
dc.language.isoeng
dc.publisherFuture Medicine
dc.rights.accessRightsopen access
dc.subject.cdu577.1
dc.subject.cdu616.24-00.5:617.7
dc.subject.keywordCellular uptake
dc.subject.keywordCorneal permeability
dc.subject.keywordCytotoxicity
dc.subject.keywordIn vitro release
dc.subject.keywordIn vivo ocular toxicity
dc.subject.keywordMinimum inhibitory concentration
dc.subject.keywordMicroemulsification
dc.subject.keywordOcular tissue uptake
dc.subject.keywordOcular tuberculosis
dc.subject.keywordStability studies
dc.subject.ucmBioquímica (Química)
dc.subject.ucmAnatomía ocular
dc.titleSolid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics
dc.typejournal article
dc.volume.number14
dspace.entity.typePublication
relation.isAuthorOfPublicationd1e44010-b3d9-4270-892d-a1f97a4db789
relation.isAuthorOfPublication.latestForDiscoveryd1e44010-b3d9-4270-892d-a1f97a4db789

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