Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples

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dc.contributor.authorLuque Uría, Álvaro
dc.contributor.authorPeltomaa, Riikka
dc.contributor.authorNavarro Duro, Marina
dc.contributor.authorFikacek, Sabrina
dc.contributor.authorHead, Trajen
dc.contributor.authorDeo, Sapna K.
dc.contributor.authorDaunert, Sylvia
dc.contributor.authorBenito Peña, María Elena
dc.contributor.authorMoreno Bondi, María Cruz
dc.date.accessioned2023-06-22T10:41:06Z
dc.date.available2023-06-22T10:41:06Z
dc.date.issued2022-02-17
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2022)
dc.description.abstractThe development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/71254
dc.identifier.doi10.1002/anse.202100070
dc.identifier.issn2629-2742
dc.identifier.officialurlhttps://doi.org/10.1002/anse.202100070
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71361
dc.journal.titleAnalysis & Sensing
dc.language.isoeng
dc.publisherWiley-VCH
dc.relation.projectID(RTI2018-096410-B-C21); (BES-2016-078137)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu543
dc.subject.keywordbioluminescence
dc.subject.keywordfumonisin B1
dc.subject.keywordfusion proteins
dc.subject.keywordimmunoassays
dc.subject.keywordluciferases
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleComparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
dc.typejournal article
dspace.entity.typePublication
relation.isAuthorOfPublicationebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85
relation.isAuthorOfPublication8766057b-6628-4a02-a6db-20bddfaf3054
relation.isAuthorOfPublication.latestForDiscoveryebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85
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