Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy

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dc.contributor.authorVelloso Álvarez, Ana
dc.contributor.authorJose Cunilleras, E.
dc.contributor.authorDorrego Rodríguez, Abel
dc.contributor.authorSantiago Llorente, Isabel
dc.contributor.authorDe la Cuesta Torrado, María
dc.contributor.authorTroya Portillo, Lucas
dc.contributor.authorRivera Arroyo, Belén
dc.contributor.authorVitale, Valentina
dc.contributor.authorJuan Ferré, Lucía De
dc.contributor.authorCruz López, Fátima
dc.date.accessioned2024-05-23T14:14:14Z
dc.date.available2024-05-23T14:14:14Z
dc.date.issued2023-09-12
dc.description2023 Acuerdos transformativos CRUE
dc.description.abstractBackground: Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak. Objectives: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS. Study design: Descriptive study of natural infection. Methods: Urine and whole blood/NS samples were collected at different time points during the hospitalisation of 21 horses involved in two EHV-1 myeloencephalopathy outbreaks in 2021 and 2023 in Spain. Quantitative real-time PCR was performed to compare the viral DNA load between BC-urine samples in 2021 and NS-urine samples in 2023. Sex, age, breed, presence of neurological signs, EHV-1 vaccination status and treatment data were recorded for all horses. Results: A total of 18 hospitalised horses during the 2021 and 2023 outbreaks were positive for EHV-1, and viral DNA was detected in urine samples from a total of 11 horses in both outbreaks. Compared with BC samples, DNA presence was detected in urine samples for longer duration and with slightly higher concentration; however, compared with NS, detection of EHV-1 in urine was similar in duration with lower DNA concentrations. Main limitations: Limited sample size, different sampling times and protocols (BC vs. NS) in two natural infection outbreak settings. Conclusions: EHV-1 was detected in the urine from naturally infected horses. Urine should be considered as complimentary to blood and NS in diagnosis of EHV-1 infection.en
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedFALSE
dc.description.statuspub
dc.identifier.citationVelloso-Alvarez A, Jose-Cunilleras E, Dorrego-Rodriguez A, Santiago-Llorente I, de las Cuesta-Torrado M, Troya-Portillo L, Rivera B, Vitale V, de Juan L and Cruz F*. Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy. Equine veterinary journal, 56(3):456-463. 2024. (A). ISSN: 0425-1644. Impact factor 2022: 2.200. Category: Veterinary Sciences, Quartile: 2, Position: 38 of 143. DOI: 10.1111/evj.14007
dc.identifier.doi10.1111/evj.14007
dc.identifier.essn2042-3306
dc.identifier.issn0425-1644
dc.identifier.officialurlhttps://doi.org/10.1111/evj.14007
dc.identifier.pmid37699794
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/37699794/
dc.identifier.relatedurlhttps://beva.onlinelibrary.wiley.com/doi/10.1111/evj.14007
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104378
dc.issue.number3
dc.journal.titleEquine Veterinary Journal
dc.language.isoeng
dc.page.final463
dc.page.initial456
dc.publisherWiley
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.1.09:616
dc.subject.keywordDiagnosis
dc.subject.keywordEHV-1
dc.subject.keywordHorse
dc.subject.keywordOutbreak
dc.subject.keywordPCR
dc.subject.keywordUrine
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleDetection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathyen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number56
dspace.entity.typePublication
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