Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples

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dc.contributor.authorCoral Polo
dc.contributor.authorGarcía-Seco Romero, María Teresa
dc.contributor.authorGarcía Benzaquén, Nerea
dc.contributor.authorVíctor Fernández
dc.contributor.authorBriones Dieste, Víctor
dc.contributor.authorDíez Guerrier, Alberto Antoine
dc.contributor.authorÁlvarez Sánchez, Julio
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorPérez Sancho, Marta
dc.date.accessioned2024-04-16T14:11:52Z
dc.date.available2024-04-16T14:11:52Z
dc.date.issued2024-04-10
dc.description.abstractThe isolation of Campylobacter fetus subsp. venerealis (Cfv) from clinical samples is the gold standard for confirming cases of bovine genital campylobacteriosis, an important cause of infertility in cattle and a potential public health concern. Furthermore, isolation is also necessary for the development of autologous vaccines, characterization of strains for antimicrobial susceptibility patterns, etc. Nevertheless, the sensitivity of culture methods is usually low, and there is no standardized protocol to maximize the recovery of Cfv from clinical samples. The aim of the current study is to design a protocol for the culture of Cfv from preputial samples by evaluating the combination of different transport, enrichment and culture media considering the impact of certain factors (time between collection and enrichment, temperature, and use of filters). The use of modified Lander's transport medium and storing the sample for 24 h at 21 ± 2 °C led to the highest recovery of Cfv CFUs. In contrast, the storage of the samples during 24-48 h in PBS and Thomann rarely allowed the recovery of Cfv regardless of the temperature. The enrichment medium yielding the best results was Preston (significantly higher recovery than Brucella medium), while Cfv could not be isolated with Bolton. Regarding our diagnostic assay (using Lander as transport medium and Preston as enrichment medium), the best protocol in terms of maximizing Cfv recovery as well as limiting contaminations is to culture the samples in i) solid media Preston or Skirrow, and ii) using 0.65 μm filters and incubating plates at 37 °C in microaerophilic conditions.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipCRUE Universidades Españolas
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationPolo C, Garcia-Seco T, Garcia N*, Fernandez-Benito V, Briones V, Diez-Guerrier A, Alvarez J, Dominguez L and Perez-Sancho M. Time, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples. Veterinary Research Communications, 1-11. 2024. (A). ISSN: 0165-7380. Impact factor 2022: 2.200. Category: Veterinary Sciences, Quartile: 2, Position: 38 of 143. DOI: 10.1007/s11259-024-10362-8
dc.identifier.doi10.1007/s11259-024-10362-8
dc.identifier.essn1573-7446
dc.identifier.issn0165-7380
dc.identifier.officialurlhttps://link.springer.com/journal/11259
dc.identifier.pmid38598116
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/38598116/#full-view-affiliation-5
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103156
dc.journal.titleVeterinary Research Communications
dc.language.isoeng
dc.page.final11
dc.page.initial1
dc.relation.projectIDind2018
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordCampylobacter fetus subsp. venerealis
dc.subject.keywordIsolation
dc.subject.keywordCulture
dc.subject.keywordTransport medium
dc.subject.keywordEnrichment medium
dc.subject.keywordPreputial bull samples
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleTime, temperature and media: the three keys to improve the recovery of Campylobacter fetus subsp. venerealis from preputial bull samples
dc.typejournal article
dc.type.hasVersionAM
dspace.entity.typePublication
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