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Thymus aging in mice deficient in either EphB2 or EphB3, two master regulators of thymic epithelium development

dc.contributor.authorGarcia-Ceca Hernández, Javier
dc.contributor.authorMontero-Herradón, Sara
dc.contributor.authorZapata González, Agustín
dc.date.accessioned2023-06-16T15:24:50Z
dc.date.available2023-06-16T15:24:50Z
dc.date.issued2020-10-20
dc.description.abstractBackground: The epithelial microenvironment is involved in thymus aging, but the possible role of EphB receptors that govern the thymic epithelium development has not been investigated. Herein, we study the changes undergone by the thymus of EphB-deficient mice throughout their life. Results: Immune alterations occurring throughout life were more severe in mutant than in wild-type (WT) mice. Mutant thymuses exhibit lower cellularity than WT ones, as well as lower proportions of early thymic progenitors cells and double-positive (CD4+CD8+) thymocytes, but higher of double-negative (CD4−CD8−) and single-positive (CD4+CD8−, CD4−CD8+) cells. Throughout life, CD4+ naïve cells decreased particularly in mutant mice. In correlation, memory T cells, largely CD8+ cells, increased. Aged thymic epithelium undergoes changes including appearance of big epithelial free areas, decrease of K8+K5− areas, which, however, contain higher proportions of Ly51+UEA1− cortical epithelial cells, in correlation with reduced Aire+ medullary epithelial cells. Also, aged thymuses particularly those derived from mutant mice exhibited increased collagen IV, fat-storing cells, and connective cells. Conclusions: The absence of EphB accelerates the alterations undergone throughout life by both thymic epithelium and thymocytes, and the proportions of peripheral naïve and memory T cells, all of which are hallmarks of immune aging.
dc.description.departmentDepto. de Biología Celular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (MCIU)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/62416
dc.identifier.doi10.1002/dvdy.212
dc.identifier.issn1058-8388; ESSN: 1097-0177
dc.identifier.officialurlhttps://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/dvdy.212?af=R
dc.identifier.urihttps://hdl.handle.net/20.500.14352/6614
dc.issue.number10
dc.journal.titleDevelopmental Dynamics
dc.language.isoeng
dc.page.final1248
dc.page.initial1243
dc.publisherWiley
dc.relation.projectID(BFU2013-41112-R)
dc.relation.projectID(RTI2018-093938-B-I00)
dc.rights.accessRightsrestricted access
dc.subject.cdu577.27
dc.subject.cdu611.438
dc.subject.keywordSenescence
dc.subject.keywordThymic epithelial cells
dc.subject.keywordThymocytes
dc.subject.ucmInmunología
dc.subject.ucmBiología celular (Biología)
dc.subject.unesco2412 Inmunología
dc.subject.unesco2407 Biología Celular
dc.titleThymus aging in mice deficient in either EphB2 or EphB3, two master regulators of thymic epithelium development
dc.typejournal article
dc.volume.number249
dspace.entity.typePublication
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