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Biosynthesis of selenium nanoparticles by Azoarcus sp. CIB

dc.contributor.authorFernández-Llamosas, Helga
dc.contributor.authorCastro Ruiz, Laura
dc.contributor.authorBlázquez Izquierdo, María Luisa
dc.contributor.authorDíaz, Eduardo
dc.contributor.authorCarmona, Manuel
dc.date.accessioned2023-12-11T14:58:20Z
dc.date.available2023-12-11T14:58:20Z
dc.date.issued2016
dc.description.abstractBackground: Different bacteria have been reported so far that link selenite resistance to the production of metallic selenium nanoparticles (SeNPs). Although SeNPs have many biotechnological applications in diverse areas, the molecular mechanisms involved in their microbial genesis are not fully understood. The Azoarcus genus is a physiologically versatile group of beta-proteobacteria of great environmental relevance. Azoarcus sp. CIB is a facultative anaerobe that combines the ability to degrade under aerobic and/or anaerobic conditions a wide range of aromatic compounds, including some toxic hydrocarbons such as toluene and m-xylene, with an endophytic life style in the root of rice. We unravel here an additional physiological feature of the strain CIB that is related to its resistance to selenium oxyanions and the formation of SeNPs. Results: This work is the first report of a member of the Azoarcus genus that is able to anaerobically grow in the presence of selenite. Electron microscopy preparations and X-ray spectroscopy analyses demonstrate the reduction of selenite to spherical electron-dense SeNPs whose average size was 123 ± 35 nm of diameter. Our data suggest that the main molecular mechanism of selenite resistance resides on an energy-dependent selenite exporter. Azoarcus cells trigger the synthesis of SeNPs when they reach the stationary-phase of growth, and either the exhaustion of electron donor or acceptor, both of which lead to starvation conditions, produce the reduction of selenite to red elemental selenium. Azoarcus becomes a promising biocatalyst, either as whole cells or cellular extracts, for the anaerobic and/or aerobic green synthesis of SeNPs. Conclusions: Azoarcus turns out to be a new eco-friendly system to reduce selenite and produce spherical SeNPs. Moreover, this is the first report of a rice endophyte able to produce SeNPs. Since Azoarcus is also able to degrade both aerobically and anaerobically toxic aromatic compounds of great environmental concern, it becomes a suitable candidate for a more sustainable agricultural practice and for bioremediation strategies.
dc.description.departmentDepto. de Ingeniería Química y de Materiales
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.identifier.citationFernández-Llamosas, H., Castro, L., Blázquez, M. L., Díaz, E., & Carmona, M. (2016). Biosynthesis of selenium nanoparticles by Azoarcus sp. CIB. Microbial cell factories, 15(1), 1-10.
dc.identifier.doi10.1186/s12934-016-0510-y
dc.identifier.issn1475-2859
dc.identifier.officialurlhttps://doi.org/10.1186/s12934-016-0510-y
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91147
dc.issue.number1
dc.journal.titleMicrobial Cell Factories
dc.language.isoeng
dc.page.final10
dc.page.initial1
dc.publisherBioMed Central
dc.relation.projectIDMAT2014-59222R
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BIO2012-39501/ES/ESTUDIO DE LOS MECANISMOS MOLECULARES QUE CONTROLAN LA ADAPTACION BACTERIANA PARA LA DEGRADACION ANAEROBICA DE COMPUESTOS AROMATICOS/
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu577.1
dc.subject.cdu612.015
dc.subject.keywordNanoparticles
dc.subject.keywordSelenium
dc.subject.keywordBioremediation
dc.subject.keywordAzoarcus
dc.subject.keywordRice
dc.subject.keywordNanotechnology
dc.subject.keywordBiotechnology
dc.subject.ucmMetalurgia
dc.subject.ucmMateriales
dc.subject.unesco3303 Ingeniería y Tecnología Químicas
dc.titleBiosynthesis of selenium nanoparticles by Azoarcus sp. CIB
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number15
dspace.entity.typePublication
relation.isAuthorOfPublication93b5b649-29f0-4442-b669-6ee59981ae2b
relation.isAuthorOfPublication7899c2ed-268c-4693-973f-e26749d0fa55
relation.isAuthorOfPublication.latestForDiscovery93b5b649-29f0-4442-b669-6ee59981ae2b

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