Publication:
Oligodendroglia generated from adult rat adipose tissue by direct cell conversion

dc.contributor.authorVellosillo, Lara
dc.contributor.authorPascual Guerra, Jorge
dc.contributor.authorPaz Muñoz, María
dc.contributor.authorRodríguez Navarro, José A.
dc.contributor.authorGonzález Nieto, Daniel
dc.contributor.authorBarrio, Luis Carlos
dc.contributor.authorToledo Lobo, Maria del Val
dc.contributor.authorPaíno, Carlos Luis
dc.date.accessioned2023-06-22T10:52:42Z
dc.date.available2023-06-22T10:52:42Z
dc.date.issued2022-02-11
dc.description.abstractObtaining oligodendroglial cells from dispensable tissues would be of great interest for autologous or immunocompatible cell replacement therapy in demyelinating diseases, as well as for studying myelin-related pathologies or testing therapeutic approaches in culture. We evaluated the feasibility of generating oligodendrocyte precursor cells (OPCs) from adult rat adipose tissue by expressing genes encoding transcription factors involved in oligodendroglial development. Adipose-derived mesenchymal cells were lentivirally transduced with tetracycline-inducible Sox10, Olig2, Zfp536, and/or Nkx6.1 transgenes. Immunostaining with the OPC-specific O4 monoclonal antibody was used to mark oligodendroglial induction. O4- and myelin-associated glycoprotein (MAG)-positive cells emerged after 3 weeks when using the Sox10 + Olig2 + Zfp536 combination, followed in the ensuing weeks by GFAP-, O1 antigen-, p75NTR (low-affinity NGF receptor)-, and myelin proteins-positive cells. The O4+ cell population progressively expanded, eventually constituting more than 70% of cells in culture by 5 months. Sox10 transgene expression was essential for generating O4+ cells but was insufficient for inducing a full oligodendroglial phenotype. Converted cells required continuous transgene expression to maintain their glial phenotype. Some vestigial characteristics of mesenchymal cells were maintained after conversion. Growth factor withdrawal and triiodothyronine (T3) supplementation generated mature oligodendroglial phenotypes, while FBS supplementation produced GFAP+ - and p75NTR+ -rich cultures. Converted cells also showed functional characteristics of neural-derived OPCs, such as the expression of AMPA, NMDA, kainate, and dopaminergic receptors, as well as similar metabolic responses to differentiation-inducing drugs. When co-cultured with rat dorsal root ganglion neurons, the converted cells differentiated and ensheathed multiple axons. We propose that functional oligodendroglia can be efficiently generated from adult rat mesenchymal cells by direct phenotypic conversion.
dc.description.departmentDepto. de Biología Celular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipFondo Social Europeo (FSE)
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipProyecto VEXEM
dc.description.sponsorshipEquilab S.A. (Madrid, Spain)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/73937
dc.identifier.doi10.3389/fcell.2022.741499
dc.identifier.issnElectronic: 2296-634X
dc.identifier.officialurlhttps://doi.org/10.3389/fcell.2022.741499
dc.identifier.relatedurlhttps://www.frontiersin.org/articles/10.3389/fcell.2022.741499/full
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71825
dc.issue.number741499
dc.journal.titleFrontiers in Cell and Developmental Biology
dc.language.isoeng
dc.page.final23
dc.page.initial1
dc.publisherFrontiers Media
dc.relation.projectID(Nos. PEJ16/MED/AI-1153 and PEJD-2018-PRE/SAL-8532)
dc.relation.projectID(SAF-2016- 78666-R, CP19-0010 and PID 2020-113014RB-I00)
dc.relation.projectID(No. S2010/BMD-2460)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu611.018.8
dc.subject.keywordADSC
dc.subject.keywordOligodendrocyte precursor cells
dc.subject.keywordMyelination
dc.subject.keywordBenztropine
dc.subject.keywordAdult rat
dc.subject.keywordDirect lineage conversion
dc.subject.ucmNeurociencias (Biológicas)
dc.subject.unesco2490 Neurociencias
dc.titleOligodendroglia generated from adult rat adipose tissue by direct cell conversion
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
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