OFF-ON on-the-fly aptassay for rapid and accurate determination of procalcitonin in very low birth weight infants with sepsis suspicion

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dc.contributor.authorGordón Pidal, José M.
dc.contributor.authorArruza Gómez, Luis
dc.contributor.authorMoreno Guzmán, María
dc.contributor.authorLópez, Miguel Ángel
dc.contributor.authorEscarpa, Alberto
dc.date.accessioned2024-01-15T10:46:19Z
dc.date.available2024-01-15T10:46:19Z
dc.date.issued2023-03-01
dc.description.abstractA fast and reliable OFF-ON micromotors (MM)-based aptassay for rapid, sensitive, and accurate determination of procalcitonin (PCT) as one of the leading neonatal sepsis biomarkers in very low birth weight infants’ serum samples is proposed. MM were composed of three specific functional layers: graphene oxide (GO) as sensing layer, Ni for magnetic guidance and platinum nanoparticles (PtNPs) as catalytic layer for self-propulsion (MMGO/Ni/PtNPs). The OFF-ON strategy relies on the binding of the aptamer tagged with alexa-405 as fluorescent label (AptPCT, λem=447 nm) onto the GO outer layer of the MM (GOMM) to induce its fluorescence quenching (GOMM-AptPCT, OFF state); which is next recovered due to selectively binding of the aptamer to the PCT and their separation from the GOMM surface (PCT-AptPCT, ON state). The fast OFF-ON on-the-fly aptassay allowed in just 5 min a highly selective and sensitive (LOD = 0.01 ng mL−1) PCT determination in clinical samples from very low birth weight infants with sepsis suspicion, using only 25 µL of sample in a clinically relevant range of concentrations (0.03–1280 ng mL−1). The high concordance between the PCT levels obtained by our MMGO/Ni/PtNPs-based aptassay and those obtained by the Hospital, demonstrated the analytical potency of our approach for the analysis of neonatal samples as well as its potential to be used as a pre-diagnostic tool for sepsis.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.doi10.1016/j.snb.2022.133107
dc.identifier.issn0925-4005
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0925400522017506
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93012
dc.journal.titleSensors and Actuators B: Chemical
dc.language.isoeng
dc.page.final133107
dc.page.initial133107
dc.publisherElsevier
dc.rights.accessRightsopen access
dc.subject.ucmCiencias
dc.subject.unesco2301 Química Analítica
dc.titleOFF-ON on-the-fly aptassay for rapid and accurate determination of procalcitonin in very low birth weight infants with sepsis suspicion
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number378
dspace.entity.typePublication
relation.isAuthorOfPublication72ff9cec-e2c8-4c06-bbf1-1ae181ebd692
relation.isAuthorOfPublication96968530-a80d-4987-a341-f4a96697f66c
relation.isAuthorOfPublication.latestForDiscovery72ff9cec-e2c8-4c06-bbf1-1ae181ebd692
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