Ghrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus)

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dc.contributor.authorBlanco Imperiali, Ayelén M.
dc.contributor.authorBertucci, Juan I.
dc.contributor.authorValenciano González, Ana Isabel
dc.contributor.authorDelgado, María Jesús
dc.contributor.authorUnniappan, Suraj
dc.date.accessioned2023-06-17T22:03:58Z
dc.date.available2023-06-17T22:03:58Z
dc.date.issued2017-07
dc.description.abstractGhrelin is an important gut-derived hormone with an appetite stimulatory role, while most of the intestinal hormones, including cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1), are appetite-inhibitors. Whether these important peptides with opposing roles on food intake interact to regulate energy balance in fish is currently unknown. The aim of this study was to characterize the putative crosstalk between ghrelin and CCK, PYY and GLP-1 in goldfish (Carassius auratus). We first determined the localization of CCK, PYY and GLP-1 in relation to ghrelin and its main receptor GHS-R1a (growth hormone secretagogue 1a) in the goldfish intestine by immunohistochemistry. Colocalization of ghrelin/GHS-R1a and CCK/PYY/GLP-1 was found primarily in the luminal border of the intestinal mucosa. In an intestinal explant culture, a significant decrease in prepro-cck, prepro-pyy and proglucagon transcript levels was observed after 60 min of incubation with ghrelin, which was abolished by preincubation with the GHS-R1a ghrelin receptor antagonist [D-Lys3]-GHRP-6 (except for proglucagon). The protein expression of PYY and GLP-1 was also downregulated by ghrelin. Finally, intraperitoneal co-administration of CCK, PYY or GLP-1 with ghrelin results in no modification of food intake in goldfish. Overall, results of the present study show for the first time in fish that ghrelin exerts repressive effects on enteric anorexigens. It is likely that these interactions mediate the stimulatory effects of ghrelin on feeding and metabolism in fish.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipNatural Sciences and Engineering Research Council (NSERC) of Canada
dc.description.sponsorshipCanada Foundation for Innovation to S. Unniappan
dc.description.sponsorshipSaskatchewan Health Research Foundation (SHRF)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44313
dc.identifier.doi10.1016/j.yhbeh.2017.05.004
dc.identifier.issn0018-506X
dc.identifier.officialurlhttps://www.journals.elsevier.com/hormones-and-behavior/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18008
dc.journal.titleHormones and Behavior
dc.language.isoeng
dc.page.final71
dc.page.initial62
dc.publisherElsevier
dc.relation.projectIDAGL2013-46448-C3-2-R
dc.relation.projectIDDiscovery Grant (341292-2012-RGPIN) and Discovery Accelerator Supplement (429436-2012-RGPAS)
dc.relation.projectIDJohn R. Evans Leaders Fund
dc.rights.accessRightsrestricted access
dc.subject.cdu591.1
dc.subject.cdu597.2/.5
dc.subject.keywordGhrelinGHS
dc.subject.keywordR1aCholecystokinin
dc.subject.keywordPeptide YY
dc.subject.keywordGlucagon-like peptide 1
dc.subject.keywordFood intake
dc.subject.keywordFish
dc.subject.ucmBiología
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmPeces
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2401.13 Fisiología Animal
dc.titleGhrelin suppresses cholecystokinin (CCK), peptide YY (PYY) and glucagon-like peptide-1 (GLP-1) in the intestine, and attenuates the anorectic effects of CCK, PYY and GLP-1 in goldfish (Carassius auratus)
dc.typejournal article
dc.volume.number93
dspace.entity.typePublication
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication.latestForDiscovery58727d30-dd58-40f9-962a-9bc051bfc74c
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