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Activation of NLRP3 inflammasome in liver of long evans lactating rats and its perinatal effects in the offspring after bisphenol F exposure

dc.contributor.authorLinillos Pradillo, Beatriz
dc.contributor.authorParedes Royano, Sergio Damián
dc.contributor.authorOrtiz-Cabello, María
dc.contributor.authorSchlumpf, Margret
dc.contributor.authorLichtensteiger, Walter
dc.contributor.authorVara Ameigeiras, Elena María
dc.contributor.authorFernández-Tresguerres Hernández, Jesús Ángel
dc.contributor.authorRancán, Lisa
dc.date.accessioned2024-10-21T11:05:06Z
dc.date.available2024-10-21T11:05:06Z
dc.date.issued2023
dc.description.abstractThe liver is the organ responsible for the metabolism and detoxification of BPF, the BPA analogue that is replacing it in plastic-based products. It is not known whether BPF can trigger inflammatory responses via the NLRP3 inflammasome, which plays a major role in the development of liver disease. The aim of this study was to evaluate nitrosative stress species (RNS) and NLRP3 inflammasome activation in the liver of lactating dams after BPF exposure. Moreover, it was studied whether this effect could also be observed in the liver of female and male offspring at postnatal day 6 (PND6). 36 Long Evans rats were randomly distributed according to oral treatment into three groups: Control, BPF-low dose (LBPF; 0.0365 mg/kg b.w./day) group and BPF-high dose (HBPF; 3.65 mg/kg b.w./day) group. The levels of nitrosative stress-inducing proteins (eNOS, iNOS, HO-1d), NLRP3 inflammasome components (NLRP3, PyCARD, CASP1) and proinflammatory cytokines (IL-1β, IL-18, IFN-γ and TNF-α) were measured by gene and protein expression in the liver of lactating dams and in female and male PND6 offspring. Lactating dams treated with LBPF showed a significant increase in iNOS and HO-1d, activation of NLRP3 components (NLRP3, PyCARD, CASP1) and promoted the release of proinflammatory cytokines such as IL-1β, IL-18, IFN-γ and TNF-α. Similar effects were found in female and male PND6 offspring after perinatal exposure. LBPF oral administration and perinatal exposure caused an increase of nitrosative stress markers and proinflammatory cytokines. Also, NLRP3 inflammasome activation was significantly increased in in the liver of lactating dams and PND6 offspring.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.departmentDepto. de Fisiología
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationLinillos-Pradillo, B.; Paredes, S.D.; Ortiz-Cabello, M.; Schlumpf, M.; Lichtensteiger, W.; Vara, E.; Tresguerres, J.A.F.; Rancan, L. Activation of NLRP3 Inflammasome in Liver of Long Evans Lactating Rats and Its Perinatal Effects in the Offspring after Bisphenol F Exposure. Int. J. Mol. Sci. 2023, 24, 14129. https://doi.org/ 10.3390/ijms241814129
dc.identifier.doi10.3390/ijms241814129
dc.identifier.issn1661-6596
dc.identifier.officialurlhttps://www.mdpi.com/1422-0067/24/18/14129
dc.identifier.urihttps://hdl.handle.net/20.500.14352/109144
dc.issue.number18
dc.journal.titleInternationl Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial14129
dc.publisherMDPI
dc.relation.projectIDProject ENDpoiNTs grant number 825759
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu612
dc.subject.cdu577
dc.subject.keywordBisphenol F
dc.subject.keywordRNS;
dc.subject.keywordNLRP3 inflammasome
dc.subject.keywordLiver
dc.subject.keywordOffspring
dc.subject.ucmFisiología
dc.subject.ucmBioquímica (Química)
dc.subject.unesco2302 Bioquímica
dc.subject.unesco2401.13 Fisiología Animal
dc.titleActivation of NLRP3 inflammasome in liver of long evans lactating rats and its perinatal effects in the offspring after bisphenol F exposure
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number24
dspace.entity.typePublication
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relation.isAuthorOfPublication6c2af8df-c12c-493f-9709-892dc41956f4
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relation.isAuthorOfPublication412d039f-5b44-405f-800d-ff0afb67ccd0
relation.isAuthorOfPublication.latestForDiscovery0adcad85-f6b2-46e9-9b1e-a2d54d5cb813

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