Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation

Simple item page
dc.contributor.authorBláquez Sarro, Juan Carlos
dc.contributor.authorGutiérrez Cepeda, Luna
dc.contributor.authorCrespo Castejón, Francisco
dc.contributor.authorSerres Dalmau, María Consolacion
dc.date.accessioned2024-01-31T16:04:32Z
dc.date.available2024-01-31T16:04:32Z
dc.date.issued2022-01-22
dc.description.abstractThe Purebred Spanish Horse, according to our clinical experience, is characterized by having a high number of stallions that do not meet the international commercial recommendations forequine-sperm cryopreservation. This means that artificial insemination with frozen semen from these stallions is less widespread than in other breeds. In this study, we investigated if the incorporation ofsingle-layer colloidal centrifugation prior to cryopreservation in clinical conditions could increase the number of ejaculates of Purebred Spanish stallions suitable for this processing, observing the influence of centrifugation and freezing extender protocol on post-thawed sperm motility. Using colloidal centrifugation, the percentage of ejaculates available to be frozen was increased from 35% (6/17) to71% (12/17), doubling the number of samples that could have been subjected to cryopreservation. We only found significant differences in linearity (LIN) and lateral head displacement (ALH) after5 min of incubation at 37 ◦C between colloidal and simple centrifugation processing techniques. No significant differences were found between the two different colloidal protocols in any of the variables considered. Colloidal centrifugation allowed us to obtain, from worse fresh-quality ejaculates, thawed sperm doses with similar quality to that of good-quality ejaculates. BotuCrio® produced, in general higher motility parameters and its characteristics than the other extenders analyzed, with significant differences found in comparison to Inra-Freeze® and Lac-Edta in both total (MOT) and progressive motility (PMOT) when using colloidal centrifugation and only in PMOT when applying simple centrifugation. Colloidal centrifugation optimized the efficiency of cryopreservation, as it allowed usto increase the number of ejaculates of Purebred Spanish Horses suitable to be frozen. Including these semen processing techniques in the freeze test could help to optimize equine-sperm cryopreservation protocols, especially when dealing with individuals or breeds for which initially low sperm quality prevents or limits their inclusion in sperm cryopreservation programs
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMICINN
dc.description.statuspub
dc.identifier.citationGutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation. Animals (Basel). 2023 Jan 22;13(3):382.
dc.identifier.doi10.3390/ani13030382
dc.identifier.issn2076-2615
dc.identifier.officialurlhttps://www.mdpi.com/2076-2615/13/3/382
dc.identifier.pmid36766271
dc.identifier.relatedurlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC9913238/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97316
dc.issue.number3
dc.journal.titleAnimals (based)
dc.language.isoeng
dc.page.initial382
dc.publisherMDPI
dc.relation.projectIDinfo:eu-repo/grantAgreement/MECD//AP2008-02034/ES/AP2008-02034/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordEquine sperm
dc.subject.keywordfreeze test
dc.subject.keywordColloidal centrifugation
dc.subject.keywordcryopreservation
dc.subject.ucmVeterinaria
dc.subject.unesco24 Ciencias de la Vida
dc.titleOptimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number13
dspace.entity.typePublication
relation.isAuthorOfPublication961d550d-44ef-4da6-9f2e-402c982fe5f8
relation.isAuthorOfPublicationd4b46bbe-e6cb-410e-8b74-b2361b41941d
relation.isAuthorOfPublication2b556176-6383-4ccf-a9cd-73a6ebaf9a95
relation.isAuthorOfPublication.latestForDiscovery961d550d-44ef-4da6-9f2e-402c982fe5f8
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
animals-13-00382.pdf
Size:
611.87 KB
Format:
Adobe Portable Document Format
Description:
Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation
downoload Download
Collections
Artículos