Fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills

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dc.contributor.authorTorres García, Claudia Esperanza
dc.contributor.authorGibello Prieto, Alicia
dc.contributor.authorNande Barbeitos, María Del Mar
dc.contributor.authorMartín Fernández, Margarita
dc.contributor.authorBlanco Suárez, María Ángeles
dc.date.accessioned2023-06-20T09:25:55Z
dc.date.available2023-06-20T09:25:55Z
dc.date.issued2008
dc.descriptionThe original publication is available at www.springerlink.com
dc.description.abstractSlime formation is a serious problem nowadays in the paper industry. Some enterobacteria are associated with the formation of slime deposits in paper and board mills. Detection and characterization of slime forming bacteria, belonging to the genus Enterobacter, Raoultella, and Klebsiella have been achieved by fluorescence in situ hybridization (FISH), using one probe based on the enterobacterial repetitive intergenic consensus sequence and other two rRNA targeted oligonucleotide probes. The effects of three kinds of antimicrobiological products (biocides, dispersants, and enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming enterobacteria and to select specific treatments to control microbial problems in the paper industry.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/11899
dc.identifier.citationTorres, C. Esperanza, et al. «Fluorescent in Situ Hybridization and Flow Cytometry as Tools to Evaluate the Treatments for the Control of Slime-Forming Enterobacteria in Paper Mills». Applied Microbiology and Biotechnology, vol. 78, n.o 5, abril de 2008, pp. 889-97. DOI.org (Crossref), https://doi.org/10.1007/s00253-008-1369-6.
dc.identifier.doi10.1007/s00253-008-1369-6
dc.identifier.essn1432-0614
dc.identifier.issn0175-7598
dc.identifier.officialurlhttps://doi.org/10.1007/s00253-008-1369-6.
dc.identifier.urihttps://hdl.handle.net/20.500.14352/49499
dc.issue.number5
dc.journal.titleApplied Microbiology and Biotechnology
dc.language.isospa
dc.page.final897
dc.page.initial889
dc.publisherSpringer
dc.relation.hasversionAM
dc.relation.projectIDSlimezymes (GRDI-2000-25676)
dc.relation.projectIDOdour Control (016730-FP6-2003-SME-1)
dc.relation.projectIDPROLIPAPEL (S-0505/AMB0100)
dc.rights.accessRightsopen access
dc.subject.keywordFISH
dc.subject.keywordFlow cytometry
dc.subject.keywordPapermaking
dc.subject.keywordSlime control
dc.subject.ucmAgua
dc.subject.ucmBiología molecular (Química)
dc.subject.ucmIngeniería química
dc.subject.ucmBioquímica (Química)
dc.subject.unesco2303.31 Química del Agua
dc.subject.unesco3303 Ingeniería y Tecnología Químicas
dc.titleFluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills
dc.typejournal article
dc.volume.number78
dspace.entity.typePublication
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relation.isAuthorOfPublication242709fa-ff35-4b05-a411-a8e2292841fa
relation.isAuthorOfPublicationda486399-ff98-4c10-8ac4-ed6b9bafd875
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relation.isAuthorOfPublication.latestForDiscoverya3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85
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