Fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills
dc.contributor.author | Torres García, Claudia Esperanza | |
dc.contributor.author | Gibello Prieto, Alicia | |
dc.contributor.author | Nande Barbeitos, María Del Mar | |
dc.contributor.author | Martín Fernández, Margarita | |
dc.contributor.author | Blanco Suárez, María Ángeles | |
dc.date.accessioned | 2023-06-20T09:25:55Z | |
dc.date.available | 2023-06-20T09:25:55Z | |
dc.date.issued | 2008 | |
dc.description | The original publication is available at www.springerlink.com | |
dc.description.abstract | Slime formation is a serious problem nowadays in the paper industry. Some enterobacteria are associated with the formation of slime deposits in paper and board mills. Detection and characterization of slime forming bacteria, belonging to the genus Enterobacter, Raoultella, and Klebsiella have been achieved by fluorescence in situ hybridization (FISH), using one probe based on the enterobacterial repetitive intergenic consensus sequence and other two rRNA targeted oligonucleotide probes. The effects of three kinds of antimicrobiological products (biocides, dispersants, and enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming enterobacteria and to select specific treatments to control microbial problems in the paper industry. | |
dc.description.department | Depto. de Sanidad Animal | |
dc.description.faculty | Fac. de Veterinaria | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | European Commission | |
dc.description.sponsorship | Comunidad de Madrid | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/11899 | |
dc.identifier.citation | Torres, C. Esperanza, et al. «Fluorescent in Situ Hybridization and Flow Cytometry as Tools to Evaluate the Treatments for the Control of Slime-Forming Enterobacteria in Paper Mills». Applied Microbiology and Biotechnology, vol. 78, n.o 5, abril de 2008, pp. 889-97. DOI.org (Crossref), https://doi.org/10.1007/s00253-008-1369-6. | |
dc.identifier.doi | 10.1007/s00253-008-1369-6 | |
dc.identifier.essn | 1432-0614 | |
dc.identifier.issn | 0175-7598 | |
dc.identifier.officialurl | https://doi.org/10.1007/s00253-008-1369-6. | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/49499 | |
dc.issue.number | 5 | |
dc.journal.title | Applied Microbiology and Biotechnology | |
dc.language.iso | spa | |
dc.page.final | 897 | |
dc.page.initial | 889 | |
dc.publisher | Springer | |
dc.relation.hasversion | AM | |
dc.relation.projectID | Slimezymes (GRDI-2000-25676) | |
dc.relation.projectID | Odour Control (016730-FP6-2003-SME-1) | |
dc.relation.projectID | PROLIPAPEL (S-0505/AMB0100) | |
dc.rights.accessRights | open access | |
dc.subject.keyword | FISH | |
dc.subject.keyword | Flow cytometry | |
dc.subject.keyword | Papermaking | |
dc.subject.keyword | Slime control | |
dc.subject.ucm | Agua | |
dc.subject.ucm | Biología molecular (Química) | |
dc.subject.ucm | Ingeniería química | |
dc.subject.ucm | Bioquímica (Química) | |
dc.subject.unesco | 2303.31 Química del Agua | |
dc.subject.unesco | 3303 Ingeniería y Tecnología Químicas | |
dc.title | Fluorescent in situ hybridization and flow cytometry as tools to evaluate the treatments for the control of slime-forming enterobacteria in paper mills | |
dc.type | journal article | |
dc.volume.number | 78 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | a3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85 | |
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relation.isAuthorOfPublication | da486399-ff98-4c10-8ac4-ed6b9bafd875 | |
relation.isAuthorOfPublication | 04f905d2-6294-4530-9d01-062828ddefb2 | |
relation.isAuthorOfPublication.latestForDiscovery | a3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85 |
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