Mesoporous bioactive glass/ɛ-polycaprolactone scaffolds promote bone regeneration in osteoporotic sheep

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dc.contributor.authorGómez Cerezo, María Natividad
dc.contributor.authorCasarrubios Molina, Laura
dc.contributor.authorSaiz-Pardo, M.
dc.contributor.authorOrtega, L.
dc.contributor.authorPablo, D. De
dc.contributor.authorDíaz-Güemes, I.
dc.contributor.authorFernández-Tomé, B.
dc.contributor.authorEnciso, S.
dc.contributor.authorSánchez-Margallo, F.M.
dc.contributor.authorPortolés Pérez, María Teresa
dc.contributor.authorArcos Navarrete, Daniel
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.date.accessioned2024-01-30T11:06:25Z
dc.date.available2024-01-30T11:06:25Z
dc.date.issued2019-05
dc.description.abstractMacroporous scaffolds made of a SiO2-CaO-P2O5 mesoporous bioactive glass (MBG) and ɛ-polycaprolactone (PCL) have been prepared by robocasting. These scaffolds showed an excellent in vitro biocompatibility in contact with osteoblast like cells (Saos 2) and osteoclasts derived from RAW 264.7 macrophages. In vivo studies were carried out by implantation into cavitary defects drilled in osteoporotic sheep. The scaffolds evidenced excellent bone regeneration properties, promoting new bone formation at both the peripheral and the inner parts of the scaffolds, thick trabeculae, high vascularization and high presence of osteoblasts and osteoclasts. In order to evaluate the effects of the local release of an antiosteoporotic drug, 1% (%wt) of zoledronic acid was incorporated to the scaffolds. The scaffolds loaded with zoledronic acid induced apoptosis in Saos 2 cells, impeded osteoclast differentiation in a time dependent manner and inhibited bone healing, promoting an intense inflammatory response in osteoporotic sheep. STATEMENT OF SIGNIFICANCE: In addition to an increase in bone fragility and susceptibility to fracture, osteoporosis also hinders the clinical success of endosseous implants and grafting materials for the treatment of bone defects. For the first time, macroporous scaffolds made of mesoporous bioactive glass and ε-caprolactone have been evaluated in a sheep model that mimics the osteoporosis conditions in humans. These implants fostered bone regeneration, promoting new bone formation at both the peripheral and the inner parts of the scaffolds, showing thick trabeculae and a high vascularization degree. Our results indicate that macroporous structures containing highly bioactive mesoporous glasses could be excellent candidates for the regenerative treatment of bone defects in osteoporotic patients.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipEuropean Research Council
dc.description.statuspub
dc.identifier.citationGómez-Cerezo N, Casarrubios L, Saiz-Pardo M, Ortega L, De Pablo D, Díaz-Güemes I, et al. Mesoporous bioactive glass/ɛ-polycaprolactone scaffolds promote bone regeneration in osteoporotic sheep. Acta Biomaterialia 2019;90:393–402. https://doi.org/10.1016/j.actbio.2019.04.019.
dc.identifier.doi10.1016/j.actbio.2019.04.019
dc.identifier.issn1742-7061
dc.identifier.officialurlhttps://doi.org/10.1016/j.actbio.2019.04.019
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96417
dc.language.isoeng
dc.publisherElsevier
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/MAT2016-75611-R AEI/FEDER
dc.rights.accessRightsrestricted access
dc.subject.keywordMesoporous bioactive glass
dc.subject.keywordScaffolds
dc.subject.keywordOsteoporosis
dc.subject.keywordZoledronic acid
dc.subject.keywordBone regeneration
dc.subject.ucmCiencias
dc.subject.unesco23 Química
dc.titleMesoporous bioactive glass/ɛ-polycaprolactone scaffolds promote bone regeneration in osteoporotic sheep
dc.typejournal article
dc.volume.number90
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery6e7648cb-126c-471f-8641-6c1b54cb4c72
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