First evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding
dc.contributor.author | Blanco Imperiali, Ayelén M. | |
dc.contributor.author | Gómez-Boronat, Miguel | |
dc.contributor.author | Madera, Diego | |
dc.contributor.author | Valenciano González, Ana Isabel | |
dc.contributor.author | Alonso Gómez, Ángel Luis | |
dc.contributor.author | Delgado Saavedra, María Jesús | |
dc.date.accessioned | 2023-06-17T12:27:21Z | |
dc.date.available | 2023-06-17T12:27:21Z | |
dc.date.issued | 2018-02 | |
dc.description.abstract | Nocturnin (NOC) is a unique deadenylase with robust rhythmic expression involved in the regulation of metabolic processes in mammals. Currently, the possible presence of NOC in fish is unknown. This report aimed to identify NOC in a fish model, the goldfish (Carassius auratus), and to study the possible regulation of its expression by feeding. Two partial-length cDNAs of 293 and 223 bp, named nocturnin-a (noc-a) and nocturnin-b (noc-b), were identified and found to be highly conserved among vertebrates. Both mRNAs show a similar widespread distribution in central and peripheral tissues, with higher levels detected for noc-a compared with noc-b. The periprandial expression profile revealed that noc-a mRNAs rise sharply after a meal in hypothalamus, intestinal bulb, and liver, whereas almost no changes were observed for noc-b. Food deprivation was found to exert opposite effects on the expression of both NOCs (generally inhibitory for noc-a, and stimulatory for noc-b) in the three mentioned tissues. A single meal after a 48-h food deprivation period reversed (totally or partially) the fasting-induced decreases in noc-a transcripts in all studied tissues and the increases in noc-b expression in the intestinal bulb. Together, this study offers the first report of NOC in fish and shows a high dependence of its expression on feeding and nutritional status. The differential responses to feeding of the two NOCs raise the possibility that they might be underlying different physiological mechanisms (e.g., food intake, lipid mobilization, energy homeostasis) in fish. | |
dc.description.department | Depto. de Genética, Fisiología y Microbiología | |
dc.description.faculty | Fac. de Ciencias Biológicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Economía, Industria y Competitividad (MINECO) | |
dc.description.sponsorship | Ministerio de Educación y Ciencia (MEC) | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/47533 | |
dc.identifier.doi | 10.1152/ajpregu.00241.2017 | |
dc.identifier.issn | 0002-9513 | |
dc.identifier.officialurl | https://www.physiology.org/doi/full/10.1152/ajpregu.00241.2017 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/12101 | |
dc.issue.number | 2 | |
dc.journal.title | American Journal of Physiology | |
dc.language.iso | eng | |
dc.page.final | R312 | |
dc.page.initial | R304 | |
dc.publisher | American Physiological Society | |
dc.relation.projectID | (AGL2016-74857-C3-2-R) | |
dc.rights.accessRights | restricted access | |
dc.subject.cdu | 597.5 | |
dc.subject.cdu | 591.1 | |
dc.subject.keyword | Feeding | |
dc.subject.keyword | Food deprivation | |
dc.subject.keyword | Nocturnin | |
dc.subject.keyword | Periprandrial | |
dc.subject.keyword | Teleost | |
dc.subject.ucm | Fisiología animal (Biología) | |
dc.subject.ucm | Peces | |
dc.subject.unesco | 2401.13 Fisiología Animal | |
dc.title | First evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding | |
dc.type | journal article | |
dc.volume.number | 314 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 58727d30-dd58-40f9-962a-9bc051bfc74c | |
relation.isAuthorOfPublication | 71c5047d-4eb1-48ac-86d4-21c7b2d335a7 | |
relation.isAuthorOfPublication | 48071251-a236-4607-ab33-5332c1472628 | |
relation.isAuthorOfPublication.latestForDiscovery | 58727d30-dd58-40f9-962a-9bc051bfc74c |
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