First evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding

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dc.contributor.authorBlanco Imperiali, Ayelén M.
dc.contributor.authorGómez-Boronat, Miguel
dc.contributor.authorMadera, Diego
dc.contributor.authorValenciano González, Ana Isabel
dc.contributor.authorAlonso Gómez, Ángel Luis
dc.contributor.authorDelgado Saavedra, María Jesús
dc.date.accessioned2023-06-17T12:27:21Z
dc.date.available2023-06-17T12:27:21Z
dc.date.issued2018-02
dc.description.abstractNocturnin (NOC) is a unique deadenylase with robust rhythmic expression involved in the regulation of metabolic processes in mammals. Currently, the possible presence of NOC in fish is unknown. This report aimed to identify NOC in a fish model, the goldfish (Carassius auratus), and to study the possible regulation of its expression by feeding. Two partial-length cDNAs of 293 and 223 bp, named nocturnin-a (noc-a) and nocturnin-b (noc-b), were identified and found to be highly conserved among vertebrates. Both mRNAs show a similar widespread distribution in central and peripheral tissues, with higher levels detected for noc-a compared with noc-b. The periprandial expression profile revealed that noc-a mRNAs rise sharply after a meal in hypothalamus, intestinal bulb, and liver, whereas almost no changes were observed for noc-b. Food deprivation was found to exert opposite effects on the expression of both NOCs (generally inhibitory for noc-a, and stimulatory for noc-b) in the three mentioned tissues. A single meal after a 48-h food deprivation period reversed (totally or partially) the fasting-induced decreases in noc-a transcripts in all studied tissues and the increases in noc-b expression in the intestinal bulb. Together, this study offers the first report of NOC in fish and shows a high dependence of its expression on feeding and nutritional status. The differential responses to feeding of the two NOCs raise the possibility that they might be underlying different physiological mechanisms (e.g., food intake, lipid mobilization, energy homeostasis) in fish.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Industria y Competitividad (MINECO)
dc.description.sponsorshipMinisterio de Educación y Ciencia (MEC)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/47533
dc.identifier.doi10.1152/ajpregu.00241.2017
dc.identifier.issn0002-9513
dc.identifier.officialurlhttps://www.physiology.org/doi/full/10.1152/ajpregu.00241.2017
dc.identifier.urihttps://hdl.handle.net/20.500.14352/12101
dc.issue.number2
dc.journal.titleAmerican Journal of Physiology
dc.language.isoeng
dc.page.finalR312
dc.page.initialR304
dc.publisherAmerican Physiological Society
dc.relation.projectID(AGL2016-74857-C3-2-R)
dc.rights.accessRightsrestricted access
dc.subject.cdu597.5
dc.subject.cdu591.1
dc.subject.keywordFeeding
dc.subject.keywordFood deprivation
dc.subject.keywordNocturnin
dc.subject.keywordPeriprandrial
dc.subject.keywordTeleost
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmPeces
dc.subject.unesco2401.13 Fisiología Animal
dc.titleFirst evidence of nocturnin in fish: two isoforms in goldfish differentially regulated by feeding
dc.typejournal article
dc.volume.number314
dspace.entity.typePublication
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication71c5047d-4eb1-48ac-86d4-21c7b2d335a7
relation.isAuthorOfPublication48071251-a236-4607-ab33-5332c1472628
relation.isAuthorOfPublication.latestForDiscovery58727d30-dd58-40f9-962a-9bc051bfc74c
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