A Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol

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dc.contributor.authorLuis-Lima, Sergio
dc.contributor.authorGarcía-Contreras, Consolación
dc.contributor.authorVázquez Gómez, Marta
dc.contributor.authorAstiz, Susana
dc.contributor.authorCarrara, Fabiola
dc.contributor.authorGaspari, Flavio
dc.contributor.authorNegrín-Mena, Natalia
dc.contributor.authorJiménez-Sosa, Alejandro
dc.contributor.authorJiménez-Hernández, Hugo
dc.contributor.authorGonzález Bulnes, Antonio
dc.contributor.authorPorrini, Esteban
dc.date.accessioned2023-06-17T12:34:19Z
dc.date.available2023-06-17T12:34:19Z
dc.date.issued2018-01-12
dc.description.abstractThere is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by ~30%: CL2 = 245 ± 93 and CL1 = 308 ± 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = −47.909 + (1.176xCL1) − (0.00063968xCL12). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.
dc.description.departmentDepto. de Producción Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. FP7
dc.description.sponsorshipEuropean Cooperation in Science and Technology (COST)
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)/Programa Ramón y Cajal
dc.description.sponsorshipInsituto de Salud Carlos III (ISCIII)
dc.description.sponsorshipRed de Investigación Renal (REDINREN)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/65035
dc.identifier.doi10.3390/ijms19010232
dc.identifier.issn1422-0067
dc.identifier.officialurlhttps://doi.org/10.3390/ijms19010232
dc.identifier.relatedurlhttps://www.mdpi.com/1422-0067/19/1/232
dc.identifier.urihttps://hdl.handle.net/20.500.14352/12523
dc.issue.number1
dc.journal.titleInternational Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial232
dc.publisherMDPI
dc.relation.projectIDIMBRAIN (316137)
dc.relation.projectIDEU COST-Action BM1308 “Sharing Advances on Large Animal Models (SALAAM)”
dc.relation.projectIDRYC-2014-16573
dc.relation.projectIDCM15/00214
dc.relation.projectIDRD16/0009/0031
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordrenal function
dc.subject.keywordiohexol plasma clearance
dc.subject.keywordswine model
dc.subject.ucmGanado porcino
dc.subject.ucmProducción animal
dc.subject.unesco3104.08 Porcinos
dc.subject.unesco3104 Producción Animal
dc.titleA Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol
dc.typejournal article
dc.volume.number19
dspace.entity.typePublication
relation.isAuthorOfPublication56797f15-df44-4726-88d9-0cf57c4a4e80
relation.isAuthorOfPublication.latestForDiscovery56797f15-df44-4726-88d9-0cf57c4a4e80
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